An integration free iPSC line was generated from fibroblast obtained from the skin of an aborted fetus in feeder free conditions using episomal based vectors expressing the pluripotency factors. The cell line generated was characterized and tested for pluripotency both in vitro and in vivo by teratoma formation and differentiation into defined lineages and brain organoids. Cell line reported here is shown to be mycoplasma free.

中文翻译：

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从具有I型三等位变体的流产获得的胎儿成纤维细胞生成iPSC。

使用表达多潜能因子的基于附加基因的载体，在无饲养者的条件下，从流产胎儿皮肤获得的成纤维细胞生成无整合iPSC系。通过畸胎瘤的形成和分化为确定的谱系和脑类器官来表征生成的细胞系并在体外和体内测试其多能性。此处报道的细胞系显示无支原体。