Fibroblasts were isolated from skin biopsies of four patients diagnosed with schizophrenia and from one healthy control. Patient fibroblasts were transfected with five episomal, non-integrative reprogramming vectors to generate human induced pluripotent stem cells (iPSC). Reprogrammed iPSC showed consistent expression of several pluripotency markers, loss of expression of exogenous reprogramming vectors and ability to differentiate into all three germ layers. Additionally, iPSC maintained their normal karyotype during reprogramming. These generated cell lines can be used to study early neurodevelopmental and neuroinflammatory processes in schizophrenia in a patient-derived in vitro model.

从四名被诊断为精神分裂症的患者的皮肤活检样本和一个健康对照中分离出成纤维细胞。用五个游离的非整合型重编程载体转染患者的成纤维细胞，以生成人诱导的多能干细胞（iPSC）。重编程的iPSC显示了几种多能性标记物的一致表达，外源重编程载体的表达缺失以及分化为所有三个胚层的能力。此外，iPSC在重编程期间保持其正常的核型。这些产生的细胞系可用于在患者衍生的体外模型中研究精神分裂症的早期神经发育和神经炎症过程。