The combination of nanofibre-based barriers and anti-adhesion drugs is potentially useful for adhesion prevention after ventral surgeries. However, drug molecules exposed to the surface of barriers easily result in an initial burst that is sharp, thus limiting the anti-adhesion efficiency. In this study, we developed a sandwiched electrospun scaffold loaded with ibuprofen (Sandwich) serving as a physical barrier, as well as an effectual carrier delivering it into the injured site for enhancing anti-adhesion capability. This Sandwich scaffold exhibited significantly a reduced initial burst of drug release in the first hour and a prolonged delivery for ibuprofen over 14 days, expected to provide the long-term anti-adhesion capability. In vitro study on fibroblasts showed that incorporation of ibuprofen effectively inhibited their adhesion and proliferation, and developed Sandwich maintained the least adhesion of L-929 after 5 days of culture (<20%). For RAW 264.7 macrophages, worse cell adhesion and poorer TNF-α production of Sandwich indicated its superior anti-inflammatory effect. In summary, the sandwiched ibuprofen-loaded scaffold showed promising potential for preventing adhesion formation.

基于纳米纤维的屏障和抗粘连药物的组合可能对腹侧手术后的粘连预防有用。然而，暴露在屏障表面的药物分子容易导致初始爆发，从而限制了抗粘连效率。在这项研究中，我们开发了一种夹心式电纺支架，其中装有布洛芬（三明治）作为物理屏障，以及将其输送到受伤部位以增强抗粘连能力的有效载体。这种夹心支架在第一个小时内显着减少了药物释放的初始爆发，并在 14 天内延长了布洛芬的递送时间，有望提供长期的抗粘连能力。体外对成纤维细胞的研究表明，布洛芬的掺入有效地抑制了它们的粘附和增殖，并且在培养 5 天后，Sandwich 保持了 L-929 的最小粘附（<20%）。对于 RAW 264.7 巨噬细胞，Sandwich 较差的细胞粘附和较差的 TNF-α 产生表明其具有优越的抗炎作用。总之，夹心布洛芬负载支架显示出防止粘连形成的潜力。