Abstract Semi-passive sulphate reducing bioreactors show potential to play an important role in remediation of persistent, low volume acid or neutral rock drainage, characterised by high sulphate concentration. The hybrid linear flow channel reactor (LFCR) integrates biological sulphate reduction and partial sulphide oxidation within a single operating unit, allowing elemental sulphur recovery. Here, the impact of reactor geometry and scale-up of the hybrid LFCR was evaluated. In addition, the use of acetate as an alternative carbon source and electron donor to lactate was investigated. Scale up from 2 to 8 L did not significantly affect process performance over the range of hydraulic residence times tested. A decrease in HRT from 4 to 2 days resulted in an increase in VSRR (2 L: 0.068 to 0.113 mmol/L/h; 8 L: 0.070–0.132 mmol/L/h), coupled with a small decrease in sulphate conversion (2 L: 63–52%; 8 L: 65–61%) in the lactate-fed reactors. This confirmed the consistency of performance across the reactor configurations. On using acetate as a carbon source and electron donor, under optimal conditions, a sulphate conversion efficiency of 64% and a VSRR of 0.069 mmol/L/h was comparable to its lactate-fed equivalent. Unlike the lactate-fed system, disruption or harvesting of the biofilm resulted in a significant decrease in sulphate reduction performance in the acetate system and an extended recovery time.

中文翻译：

---

反应器几何形状和电子给体对混合线性流道反应器性能的影响

摘要 半被动硫酸盐还原生物反应器显示出在修复以高硫酸盐浓度为特征的持久性、低容量酸性或中性岩石排水方面发挥重要作用的潜力。混合线性流道反应器 (LFCR) 在单个操作单元内集成了生物硫酸盐还原和部分硫化物氧化，从而可以回收元素硫。在这里，评估了反应器几何形状和混合 LFCR 放大的影响。此外，还研究了使用乙酸盐作为替代碳源和电子供体的乳酸盐。在测试的水力停留时间范围内，从 2 L 放大到 8 L 不会显着影响工艺性能。HRT 从 4 天减少到 2 天导致 VSRR 增加（2 L：0.068 至 0.113 mmol/L/h；8 L：0.070–0.132 mmol/L/h），再加上乳酸反应器中硫酸盐转化率的小幅下降（2 L：63-52%；8 L：65-61%）。这证实了反应器配置之间性能的一致性。在使用乙酸盐作为碳源和电子供体时，在最佳条件下，64% 的硫酸盐转化效率和 0.069 mmol/L/h 的 VSRR 与其喂食的乳酸盐相当。与乳酸喂养系统不同，生物膜的破坏或收获导致醋酸盐系统中硫酸盐还原性能的显着降低和恢复时间延长。64% 的硫酸盐转化效率和 0.069 mmol/L/h 的 VSRR 与乳酸喂养的等效物相当。与乳酸喂养系统不同，生物膜的破坏或收获导致醋酸盐系统中硫酸盐还原性能的显着降低和恢复时间延长。64% 的硫酸盐转化效率和 0.069 mmol/L/h 的 VSRR 与乳酸喂养的等效物相当。与乳酸喂养系统不同，生物膜的破坏或收获导致醋酸盐系统中硫酸盐还原性能的显着降低和恢复时间延长。