The determination of creatinine levels is essential for the detection of renal and muscular dysfunction. Luminescent nanoparticles are emerging as fast, cheap and highly selective sensors for the detection and quantification of creatinine. Nevertheless, current nanosensors only have a short shelf life due to their poor chemical and colloidal stability, which limits their clinical functionality. In this work, we have developed a highly stable, selective and sensitive nanosensor based on europium-doped, amorphous calcium phosphate nanoparticles (Eu-ACP) for the determination of creatinine by luminescence spectroscopy. The colloidal stability of Eu-ACP nanoparticles in aqueous solutions was optimised to ensure a constant signal after up to 4 months in storage. The luminescence intensity of Eu-ACP decreased linearly with the creatinine concentration over the range of 1-120 μM (R² = 0.995). This concentration–response relationship was used to determine creatinine levels in real urine samples resulting in good recovery percentages. Significantly, selectivity assays indicated that none of the potential interfering species provoked discernible changes in the luminescence intensity.

肌酐水平的测定对于检测肾脏和肌肉功能障碍至关重要。发光纳米粒子正以快速，廉价和高度选择性的传感器出现，用于肌酐的检测和定量。然而，当前的纳米传感器由于其差的化学和胶体稳定性而仅具有短的保存期限，这限制了它们的临床功能。在这项工作中，我们开发了一种基于ped掺杂无定形磷酸钙纳米粒子（Eu-ACP）的高度稳定，选择性和灵敏的纳米传感器，用于通过发光光谱法测定肌酐。优化了Eu-ACP纳米粒子在水溶液中的胶体稳定性，以确保在储存长达4个月后保持恒定的信号。² = 0.995）。这种浓度-反应关系用于确定实际尿液样本中的肌酐水平，从而导致良好的回收率。重要的是，选择性测定表明没有潜在的干扰物质引起发光强度的明显变化。