Although many entomogenous fungi have been discovered over the years, few studies on the crude extracts of fungi isolated from Cryptotympana atrata with antibacterial and antifungal activity were reported. In this study, total twenty entomogenous fungi were isolated for the first time. And among of them, two pure cultures were identified as Purpureocillium lilacinum and Aspergillus fumigatus with apparent morphology, microscopic identification and 18S rRNA gene sequence. The active strains were fermented to optimize in six different culture media at three different pH values. The antibacterial and antifungal activities of the metabolites were more potent and efficient in Fungal medium 3^# at a pH of 6.2 than in the other tested media or at the other tested pH values. Total seven human pathogens and one insect pathogen were used to evaluate the antibacterial and antifungal activity of crude extracts, among which 25% of the extracts exhibited antifungal activity against Verticillium lecanii, while 33.3% and 47.2% of the extracts exhibited antibacterial activity against the important human pathogens Staphylococcus aureus and Bacillus cereus, respectively. The range of the MICs was from 15.6 to 250 μg mL⁻¹, and 35% of the fungal metabolites exhibited antibacterial activity against Pseudomonas aeruginosa, Bacillus thuringiensis and Enterobacter aerogenes at 1000 μg mL⁻¹ except the previously described antibacterial activities. Furthermore, the phylogenetic relationships of the two identified fungi were also constructed. In brief, it is the first reporting about enthompathogenic fungi from Cryptotympana atrata and provides candidate strains with potential use as biological agents and against multidrug-resistant organisms.

尽管多年来已发现许多昆虫致病真菌，但很少有人对从隐 孢子虫分离的具有抗菌和抗真菌活性的真菌的粗提物进行研究。在这项研究中，首次分离出总共二十种昆虫致病真菌。和它们之间，两个纯培养物鉴定为Purpureocillium lilacinum和曲霉属 霉菌与表观形貌，显微鉴定和18S rRNA基因序列。在三种不同的pH值下，在六种不同的培养基中对活性菌株进行发酵以进行优化。代谢产物的抗菌和抗真菌活性在3 ^＃真菌培养基中更为有效。在6.2的pH值下比在其他测试介质中或在其他测试的pH值下。总共使用了7种人类病原体和1种昆虫病原体来评估粗提物的抗菌和抗真菌活性，其中25％的提取物具有对黄萎病 菌的抗真菌活性，而33.3％和47.2％的提取物具有对重要黄萎病菌的抗菌活性。人病原体分别为金 黄色葡萄球菌和蜡状芽孢 杆菌。MIC的范围为15.6至250μgmL ^-1，并且35％的真菌代谢产物表现出对铜绿 假单胞菌，芽孢杆菌的抗菌活性 除前述抗菌活性外，苏云金杆菌和产气 肠杆菌的浓度为1000μgmL ^-1。此外，还构建了两种已鉴定真菌的系统发育关系。简言之，它是关于从enthompathogenic真菌第一报告Cryptotympana 蝉，并提供候选株生物制剂和抗多重耐药微生物的潜在用途。