The Spanish local cultivar ‘Ambrunés’ stands out due to its high organoleptic quality and fruit firmness. These characteristics make it an important parent for breeding cherries with excellent fresh and post-harvest quality. In this work, an F₁ sweet cherry population (n = 140) from ‘Ambrunés’ × ‘Sweetheart’ was phenotyped for 2 years for fruit diameter, weight and firmness and genotyped with the RosBREED cherry Illumina Infinium® 6K SNP array v1. These data were used to construct a linkage map and to carry out quantitative trait locus (QTL) mapping of these fruit quality traits. Genotyping of the parental cultivars revealed that ‘Ambrunés’ is highly heterozygous, and its genetic map is the longest reported in the species using the same SNP array. Phenotypic data analyses confirmed a high heritability of fruit size and firmness and a distorted segregation towards softer and smaller fruits. However, individuals with larger and firmer fruits than the parental cultivars were observed, revealing the presence of alleles of breeding interest. In contrast to other genetic backgrounds in which a negative correlation was observed between firmness and size, in this work, no correlation or low positive correlation was detected between both traits. Firmness, diameter and weight QTLs detected validated QTLs previously found for the same traits in the species, and major QTLs for the three traits were located on a narrow region of LG1 of ‘Ambrunés’. Haplotype analyses of these QTLs revealed haplotypes of breeding interest in coupling phase in ‘Ambrunés’, which can be used for the selection of progeny with larger and firmer fruits.

西班牙本地品种“Ambrunés”因感官品质和水果硬度高而脱颖而出。这些特性使其成为具有出色的新鲜和收获后品质的樱桃育种的重要亲本。在这项工作中，一个F ₁甜樱桃种群（n = 140）来自'Ambrunés'×'Sweetheart'的表型进行了两年的水果直径，重量和硬度测定，并使用RosBREED樱桃IlluminaInfinium®6K SNP阵列v1进行了基因分型。这些数据用于构建连锁图谱，并对这些水果品质性状进行定量性状基因座（QTL）作图。亲本品种的基因分型显示，'Ambrunés'是高度杂合的，其遗传图谱是使用相同SNP阵列的物种中报道时间最长的。表型数据分析证实了果实大小和硬度的高遗传力，以及偏向较软和较小果实的分离偏斜。但是，观察到果实比亲本品种更大和更坚硬的个体，表明存在具有育种兴趣的等位基因。与在硬度和大小之间观察到负相关的其他遗传背景相反，在这项工作中，两个性状之间均未发现相关或低正相关。硬度，直径和重量QTL检测到的是先前在该物种中具有相同性状的有效QTL，而三个性状的主要QTL位于“Ambrunés” LG1的狭窄区域。这些QTL的单倍型分析揭示了'Ambrunés'耦合阶段育种兴趣的单倍型，可用于选择果实更大，更结实的子代。这三个性状的主要QTL位于“Ambrunés” LG1的狭窄区域。这些QTL的单倍型分析揭示了'Ambrunés'耦合阶段育种兴趣的单倍型，可用于选择果实更大，更结实的子代。这三个性状的主要QTL位于“Ambrunés” LG1的狭窄区域。这些QTL的单倍型分析揭示了'Ambrunés'耦合阶段育种兴趣的单倍型，可用于选择果实更大，更结实的子代。