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Combined-evidence analyses of ultraconserved elements and morphological data: an empirical example in iguanian lizards.
Biology Letters ( IF 2.8 ) Pub Date : 2020-08-26 , DOI: 10.1098/rsbl.2020.0356
Simon G Scarpetta 1
Affiliation  

Genomic datasets generated by next-generation sequencing are increasingly prevalent in phylogenetics, but morphological data are required to phylogenetically place fossils, corroborate molecular hypotheses and date phylogenies. Combined-evidence analyses provide an integrative assessment of tree topology. However, no attempt has been made to simultaneously analyse next-generation genomic datasets and morphological data, and the future of morphology in the context of genomic data is uncertain. I conducted combined-evidence analyses that include genomic and morphological datasets, specifically, with ultraconserved elements and two morphological matrices. In unweighted maximum-likelihood and Bayesian combined-evidence analyses, morphological signal was dwarfed by the ultraconserved elements, and some node support values were reduced relative to ultraconserved element-only analyses. Increasing the weight of morphological characters allowed those data to influence the tree, but weighting subjectivity should be considered in future analyses. More attempts should be made to simultaneously analyse genomic and morphological datasets.



中文翻译:

超保守元素和形态数据的联合证据分析:鬣蜥蜥蜴的经验例子。

下一代测序生成的基因组数据集在系统发育学中越来越普遍,但需要形态学数据来对化石进行系统发育定位、证实分子假设和确定系统发育年代。联合证据分析提供了树拓扑的综合评估。然而,尚未尝试同时分析下一代基因组数据集和形态学数据,并且基因组数据背景下形态学的未来是不确定的。我进行了联合证据分析,包括基因组和形态学数据集,特别是超保守元素和两个形态学矩阵。在未加权最大似然和贝叶斯联合证据分析中,形态学信号因超保守元素而相形见绌,并且相对于仅超保守元素分析,一些节点支持值有所降低。增加形态特征的权重可以使这些数据影响树木,但在未来的分析中应考虑加权主观性。应该做出更多尝试来同时分析基因组和形态学数据集。

更新日期:2020-08-26
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