Abstract An analytical method for the accurate determination of essential fatty acids in cereals and green vegetables is established based on isotope dilution-gas chromatography/mass spectrometry (ID-GC/MS). In this approach, 13 C-labeled fatty acids were added as internal standards to the samples prior to the sample preparation. Repeatability and reproducibility for the analyses of the essential fatty acids extracted from homogenized cereals and green vegetables were examined to validate the ID-GC/MS method. The repeatability and reproducibility were within 2% relative standard deviation. The developed method provides higher-order measurement results with the relative expanded uncertainties of 1–2% and 2–4% for cereals and green vegetables, respectively. Based on the fatty acid measurements, the developed ID-GC/MS method can be used as a candidate reference method for the quantitation of essential fatty acids in cereals and green vegetables. The developed method was also applied to analyze several commercially available products including cereal and vegetable powders.

中文翻译：

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同位素稀释-气相色谱/质谱法定量分析谷物和绿色蔬菜中的必需脂肪酸

摘要 建立了一种基于同位素稀释-气相色谱/质谱（ID-GC/MS）的准确测定谷物和绿色蔬菜中必需脂肪酸的分析方法。在这种方法中，在样品制备之前，将 13 C 标记的脂肪酸作为内标添加到样品中。检查了从均质谷物和绿色蔬菜中提取的必需脂肪酸的分析的重复性和再现性，以验证 ID-GC/MS 方法。重复性和再现性在 2% 的相对标准偏差内。开发的方法提供了更高阶的测量结果，谷物和绿色蔬菜的相对扩展不确定度分别为 1-2% 和 2-4%。根据脂肪酸测量，开发的 ID-GC/MS 方法可用作谷物和绿色蔬菜中必需脂肪酸定量的候选参考方法。开发的方法还用于分析几种市售产品，包括谷物和蔬菜粉。