The stem cell is the foundation of regenerative medicine and tissue engineering. Regulating specific stem cell fate, such as cell attachment, proliferation, differentiation, and even death, undergoes continuous development. Cold atmospheric plasma (CAP), the core technology of plasma medicine, is attracting tremendous attention due to its ability and versatility to manipulate various types of cells, including stem cells. Specifically, the direct and indirect applications of CAP in controlling cell fate are best exemplified by upfront irradiation of the stem cells and modification of the stem cell niche, respectively. This review will describe the recent advances in various CAP strategies, both direct and indirect, and their influence on the fate of healthy and cancer stem cells. Particular emphasis will be placed on the mechanism of connecting the physical and chemical cues carried by the plasma and biological changes presented by the cells, especially at the transcriptomic level. The ultimate goal is to exploit CAP’s potential in regenerative medicine.

中文翻译：

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使用冷大气等离子体控制干细胞命运。

干细胞是再生医学和组织工程的基础。调节特定的干细胞命运，例如细胞附着，增殖，分化，甚至死亡，经历了不断的发展。冷血浆血浆（CAP）是血浆医学的核心技术，由于它具有操纵多种类型的细胞（包括干细胞）的能力和多功能性，因此备受关注。具体而言，CAP在控制细胞命运方面的直接和间接应用分别以干细胞的前期照射和干细胞生态位的修饰为最好。这篇综述将描述各种CAP策略（直接和间接）的最新进展，以及它们对健康和癌症干细胞命运的影响。将特别强调连接血浆所携带的物理和化学线索与细胞所呈现的生物学变化（尤其是在转录组水平上）的机制。最终目标是挖掘CAP在再生医学中的潜力。