The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes. We use long-read sequencing methods and modern scaffolding techniques (PacBio, 10X, and Hi-C) to produce AalbF2, a dramatically improved assembly of the Ae. albopictus genome. AalbF2 reveals widespread viral insertions, novel microRNAs and piRNA clusters, the sex-determining locus, and new immunity genes, and enables genome-wide studies of geographically diverse Ae. albopictus populations and analyses of the developmental and stage-dependent network of expression data. Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes. The AalbF2 genome assembly represents the most up-to-date collective knowledge of the Ae. albopictus genome. These resources represent a foundation to improve understanding of the adaptation potential and the epidemiological relevance of this species and foster the development of innovative control measures.

中文翻译：

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改进的虫媒病毒载体白纹伊蚊的参考基因组

亚洲虎蚊白纹伊蚊正在全球扩张，已成为欧洲人虫媒病毒的主要传播媒介。由于可用的抗病毒药物和疫苗有限，媒介控制是预防蚊媒疾病的主要方法。Ae 的可靠和准确的 DNA 序列。albopictus 基因组对于开发涉及蚊子基因操作的新方法至关重要。我们使用长读长测序方法和现代支架技术（PacBio、10X 和 Hi-C）来生产 AalbF2，这是一种显着改进的 Ae 组装。白纹海棠基因组。AalbF2 揭示了广泛的病毒插入、新的 microRNA 和 piRNA 簇、性别决定位点和新的免疫基因，并能够对地理上不同的 Ae 进行全基因组研究。albopictus 种群和表达数据的发育和阶段依赖网络的分析。此外，我们为该物种构建了第一个物理图谱，其中 75% 的组装基因组锚定在染色体上。AalbF2 基因组组装代表了 Ae 的最新集体知识。白纹海棠基因组。这些资源是提高对该物种适应潜力和流行病学相关性的理解并促进创新控制措施发展的基础。