The burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide but still its diagnosis is delayed and it is mistaken as multidrug-resistant tuberculosis (MDR-TB).The present study was performed to develop a multiplex PCR assay for detection and identification of clinically most common NTM to the species level from pulmonary samples. Out of 50 isolates, 26 were identified as Mycobacterium kansasii (MK), 20 were identified as Mycobacterium abscessus (MA) and 4 were identified as Mycobacterium avium complex (MAC) through multiplex PCR and further confirmed by sequencing. Our study showed that multiplex PCR assay is a simple, convenient, and reliable technique for detection and differential identification of major NTM species.

中文翻译：

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通过一步多重 PCR 检测从肺部样本中检测临床上重要的非结核分枝杆菌 (NTM)。

非结核分枝杆菌 (NTM) 疾病的负担在世界范围内不断增加，但其诊断仍被延误，并被误认为是耐多药结核病 (MDR-TB)。本研究旨在开发一种用于检测和鉴定的多重 PCR 检测方法临床上最常见的 NTM 从肺部样本到物种水平。在50株分离物中，通过多重PCR鉴定26株为堪萨斯分枝杆菌（MK），20株为脓肿分枝杆菌（MA），4株为鸟分枝杆菌复合体（MAC），并通过测序进一步确认。我们的研究表明，多重 PCR 检测是一种简单、方便且可靠的技术，可用于检测和差异鉴定主要 NTM 物种。