Pretreatment processes and subsequent enzymatic hydrolysis are prerequisites to utilize lignocellulosic sugar for fermentation. However, the resulting hydrolysate frequently hinders fermentation processes due to the presence of inhibitors and toxic products (e.g., ethanol). Thus, it is crucial to develop robust microbes conferring multi-stress tolerance. Zmo0994, a functionally uncharacterized protein from Zymomonas mobilis, was identified and characterized for the first time. A major effect of Zmo0994 was a significant enhancement in the tolerance to abiotic stresses such as ethanol, furfural, 5′-hydroxymethylfurfural and high temperature, when expressed in Escherichia coli. Through transcriptome analysis and in vivo experiments, the cellular mechanism of this protein was revealed as due to its ability to trigger genes, involved in aerobic respiration for ATP synthesis. These findings have significant implications that might lead to the development of robust microbes for the highly efficient industrial fermentation processes.

中文翻译：

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Zmo0994 是一种来自运动发酵单胞菌的新型 LEA 样蛋白，可提高大肠杆菌对多种非生物胁迫的耐受性。

预处理过程和随后的酶水解是利用木质纤维素糖进行发酵的先决条件。然而，由于存在抑制剂和有毒产物（例如乙醇），所得水解产物经常阻碍发酵过程。因此，开发具有多应力耐受性的强大微生物至关重要。Zmo0994 是一种来自运动发酵单胞菌的功能上未鉴定的蛋白质，首次被鉴定和鉴定。当在大肠杆菌中表达时，Zmo0994 的主要作用是显着增强对非生物胁迫（例如乙醇、糠醛、5'-羟甲基糠醛和高温）的耐受性。通过转录组分析和体内实验，揭示了这种蛋白质的细胞机制是因为它能够触发基因，参与ATP合成的有氧呼吸。这些发现具有重要意义，可能会导致开发用于高效工业发酵过程的强大微生物。