This study includes the design of a chitosan membrane decorated with unsaturated fatty acid–based carrier system for cancer treatment and antibacterial application. For this, polystyrene-graft-polyoleic acid-graft-polyethylene glycol was prepared by free radical polymerization and characterized. Nanoparticles and caffeic acid–loaded nanoparticles were prepared by solvent evaporation technique and optimized. The short-term stability of nanoparticles was investigated at 4°C. Drug encapsulation and loading efficiency were evaluated. The chitosan membrane and caffeic acid–loaded nanoparticles embedded into chitosan membrane were fabricated. The caffeic acid loaded nanoparticles embedded into chitosan membrane showed controlled release. The mechanical properties of all samples were investigated. The caffeic acid–loaded nanoparticles embedded into chitosan membranes indicated excellent antibacterial properties against the Escherichia coli and Staphylococcus aureus. The anticancer activity of all the samples was evaluated against SaOS-2 human primary osteogenic sarcoma and MC3T3-E1 pre-osteoblast cell lines by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, the flow cytometry and double staining methods. As a result, the designed carrier system showed great potential to cancer-associated infections treatment in bone cancer cases.

中文翻译：

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基于不饱和脂肪酸的纳米粒子改进壳聚糖膜的制备用于癌症相关感染

这项研究包括设计一种用不饱和脂肪酸基载体系统装饰的壳聚糖膜，用于癌症治疗和抗菌应用。为此，通过自由基聚合制备聚苯乙烯接枝聚油酸接枝聚乙二醇并对其进行表征。通过溶剂蒸发技术制备纳米颗粒和负载咖啡酸的纳米颗粒并进行优化。在 4°C 下研究了纳米颗粒的短期稳定性。评估药物包封和装载效率。制造了壳聚糖膜和嵌入壳聚糖膜的负载咖啡酸的纳米颗粒。嵌入壳聚糖膜的负载咖啡酸的纳米颗粒显示出受控释放。研究了所有样品的机械性能。嵌入壳聚糖膜的载有咖啡酸的纳米颗粒对大肠杆菌和金黄色葡萄球菌具有优异的抗菌性能。通过 3-[4,5-二甲基噻唑-2-基]-2,5 二苯基溴化四唑测定，评估了所有样品对 SaOS-2 人原代成骨肉瘤和 MC3T3-E1 前成骨细胞系的抗癌活性。流式细胞术和双染色法。因此，设计的载体系统在骨癌病例的癌症相关感染治疗中显示出巨大的潜力。5 二苯基溴化四唑测定法、流式细胞术和双染法。因此，设计的载体系统在骨癌病例的癌症相关感染治疗中显示出巨大的潜力。5 二苯基溴化四唑测定法、流式细胞术和双染法。因此，设计的载体系统在骨癌病例的癌症相关感染治疗中显示出巨大的潜力。