Nano silver/polyvinyl alcohol hydrogel nanocomposites have been prepared using gamma irradiation technique. Gamma irradiation serves as a crosslinking agent for the polyvinyl alcohol hydrogels and also acts as a reducing agent for reduction of Ag+ ions to zero valent Ag0 within the polyvinyl alcohol crosslinked network. The microstructural characteristics of the prepared composites were studied using powder X-ray diffraction, ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy and field emission scanning electron microscopy analysis. The data obtained by these characterizations indicate the homogeneous distribution of silver nanoparticles on the polyvinyl alcohol network. The swelling properties and mechanical parameters of the silver/polyvinyl alcohol hydrogel nanocomposites tend to show improvements, making them a better material for wound care applications. The silver/polyvinyl alcohol hydrogel nanocomposites have shown good antibacterial potential against both Gram-positive and Gram-negative bacteria and shown nil or minimal cytotoxic effect on human melanoma (SK-MEL-2) and mouse melanoma (B16-F1) cell lines. Overall, it was concluded that under optimized condition, silver/polyvinyl alcohol hydrogel nanocomposites synthesized using gamma irradiation technique are excellent candidates for wound dressing application.

中文翻译：

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用于伤口敷料应用的辐射合成银/聚乙烯水凝胶纳米复合材料的体外生物相容性评价

已经使用伽马辐射技术制备了纳米银/聚乙烯醇水凝胶纳米复合材料。伽马辐射作为聚乙烯醇水凝胶的交联剂，也作为还原剂，将聚乙烯醇交联网络内的 Ag+ 离子还原为零价 Ag0。使用粉末X射线衍射、紫外-可见光谱、傅里叶变换红外光谱和场发射扫描电子显微镜分析研究了制备的复合材料的微观结构特征。通过这些表征获得的数据表明银纳米颗粒在聚乙烯醇网络上的均匀分布。银/聚乙烯醇水凝胶纳米复合材料的溶胀性能和力学参数趋于改善，使它们成为伤口护理应用的更好材料。银/聚乙烯醇水凝胶纳米复合材料对革兰氏阳性菌和革兰氏阴性菌均显示出良好的抗菌潜力，并且对人黑色素瘤 (SK-MEL-2) 和小鼠黑色素瘤 (B16-F1) 细胞系的细胞毒作用为零或极小。总的来说，得出的结论是，在优化条件下，使用伽马辐射技术合成的银/聚乙烯醇水凝胶纳米复合材料是伤口敷料应用的优秀候选者。