A sensitive and accurate method was developed for the simultaneous determination of twelve components including phenolic acids (gallic acid, protocatechuic aldehyde, protocatechuic acid, and ferulic acid), flavonoids (catechin, epicatechin, rutin, luteolin, luteolin-7-glucoside, and epicatechin gallate), anthraquinones (emodin), and triterpenes (ursolic acid) in Cynomorii herba in different harvest times by liquid chromatography tandem mass spectrometry (LC-MS/MS). The chromatographic separation was achieved on an Eclipse plus C₁₈ (3.0 mm × 50 mm, 1.8 μm) column at 40°C. The mobile phase consisted of acetonitrile and 0.05% formic acid with a gradient elution at a flow rate of 0.4 mL·min ⁻¹. Under the optimized conditions, there was good linear relation (r ≥ 0.9944) and satisfactory precision (RSD values less than 5.65%). The recoveries of the twelve components were in the range of 93.5–105%. Moreover, the limits of detection (LOD) ranged from 0.003 to 21 ng mL⁻¹ for the twelve analytes. In conclusion, the validated method was successfully applied to analyze the change regularity of the twelve components of Cynomorii herba in different harvest times. It provides a theoretical basis for choosing the suitable harvesting time of Cynomorii herba.

中文翻译：

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通过LC-MS / MS同时测定不同收获时间的食蟹草中酚酸，蒽醌，类黄酮和三萜类化合物。

建立了一种灵敏而准确的方法，可同时测定十二种成分，包括酚酸（没食子酸，原儿茶醛，原儿茶酸和阿魏酸），类黄酮（儿茶素，表儿茶素，芦丁，木犀草素，木犀草素-7-葡萄糖苷和表儿茶素）液相色谱串联质谱法（LC-MS / MS）在不同收获时间下的食蟹草中的没食子酸酯，蒽醌（大黄素）和三萜（熊果酸）。色谱分离在Eclipse加C达到₁₈（3.0毫米×50毫米，1.8  μ M），在40℃柱。流动相由乙腈和0.05％甲酸组成，梯度洗脱为0.4 mL·min ^-1的流速。在优化条件下，线性关系良好（ř  ≥0.9944）和令人满意的精度（不包括5.65％以下RSD值）。十二种组分的回收率在93.5-105％之间。此外，十二种分析物的检出限（LOD）为0.003至21 ng mL ^-1。综上所述，验证方法成功地用于分析食蟹草在不同收获时间的十二种成分的变化规律。它为选择合适的食蟹草收获时间提供了理论依据。