Three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed via transformation of Escherichia coli (E. coli) cells with plasmids, in which a promotor of genes matA, safA, or chbB controls the expression of a fluorescent protein. The biosensors were exposed to THz radiation from two sources: a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence were found to depend on radiation parameters and exposure time. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensors are evaluated to be suitable for studying influence of THz radiation on the activity of gene networks related with considered gene promoters.

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太赫兹辐射对大肠埃希菌基因网络影响的荧光生物传感器研究

通过用质粒转化大肠杆菌（E. coli）细胞，开发了三种对太赫兹（THz）辐射敏感的新型荧光生物传感器，其中的质粒是matA，safA或chbB基因的启动子控制荧光蛋白的表达。生物传感器受到来自两个来源的太赫兹辐射的影响：高强度脉冲短波自由电子激光器和低强度连续长波IMPATT二极管基设备。发现荧光的阈值和动力学取决于辐射参数和曝光时间。热激或化学应力导致没有荧光诱导。经评估，该生物传感器适合研究太赫兹辐射对与考虑的基因启动子有关的基因网络活性的影响。