Accelerated Green Process of 2,5-Dimethylpyrazine Production from Glucose by Genetically Modified Escherichia coli.,ACS Synthetic Biology

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Accelerated Green Process of 2,5-Dimethylpyrazine Production from Glucose by Genetically Modified Escherichia coli.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-08-25 , DOI: 10.1021/acssynbio.0c00329
Jianzhong Xu ₁ , Haibo Yu ₁ , Xiulai Chen ₂ , Liming Liu ₂ , Weiguo Zhang ₁

Affiliation

2,5-Dimethylpyrazine (2,5-DMP) is an indispensable additive for flavoring in the food industry and an important substrate for producing hypoglycemic and antilipolytic drugs. However, 2,5-DMP is produced by chemical synthesis in industry. Herein, a “green” strategy to produce 2,5-DMP has been reported for the first time. To do this, we rewrote the de novo 2,5-DMP biosynthesis pathway and substrate transmembrane transport in an l-threonine high-yielding strain to promote highly efficient 2,5-DMP production from glucose by submerged fermentation. The final strain T6-47-7 could produce 1.43 ± 0.07 g/L of 2,5-DMP with a carbon yield of 6.78% and productivity of 0.715 g/(L·d) in shake-flask fermentation using a phase-wise manner of hypoxia-inducible expression. The design-based strategy for constructing the 2,5-DMP high-yielding strain reported here could serve as a general concept for breeding high-yielding strains that produce some other type of alkylpyrazine.

中文翻译：

转基因大肠杆菌加速葡萄糖生产 2,5-二甲基吡嗪的绿色工艺。

2,5-二甲基吡嗪（2,5-DMP）是食品工业中不可缺少的调味添加剂，也是生产降血糖和抗脂解药物的重要底物。然而，2,5-DMP在工业上是通过化学合成生产的。在此，首次报道了生产2,5-DMP的“绿色”策略。为此，我们重写了l-苏氨酸高产菌株中的从头2,5-DMP生物合成途径和底物跨膜运输，以促进通过深层发酵从葡萄糖高效生产2,5-DMP。最终菌株T6-47-7在分相摇瓶发酵中可产生1.43 ± 0.07 g/L 2,5-DMP，碳产率为6.78%，生产率为0.715 g/(L·d)。缺氧诱导表达的方式。本文报道的基于设计的构建 2,5-DMP 高产菌株的策略可以作为培育产生某些其他类型烷基吡嗪的高产菌株的一般概念。

更新日期：2020-09-20

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