Background: In pollinosis patients, allergen-specific antibody titers show seasonal variations. Little is known about these variations at the epitope level. Objectives: We aimed at investigating seasonal variations on the level of allergen epitope recognition in patients with Bet v 1-related food allergy using a peptide phage display approach. Methods: Serum samples collected over 1 year from 4 patients of the placebo arm of the birch-associated soya allergy immunotherapy trial were included. To identify epitopes from Bet v 1-related food allergens, patient sera were used in peptide phage display experiments. In silico analysis of enriched allergen-related motifs was performed. Results: We identified epitope motifs related to Bet v 1 and its homologs in soya and hazelnut (Gly m 4 and Cor a 1, respectively) that were enriched in accordance with birch and hazel pollen exposure. Within several weeks after the birch pollen season peak, the pattern of identified epitope motifs differed considerably among patients. Data for amino acid preferences in homologous Bet v 1 and Cor a 1 epitope motifs identified for one of the investigated patients suggest changes in concentration or specificity of serum antibodies for the Cor a 1 epitope motif. Conclusions: Peptide phage display data suggest an impact of birch and hazel pollen exposure on the recognition pattern of Bet v 1-like allergen epitopes. Epitope-oriented analyses could provide deeper, personalized details regarding the allergen epitope recognition influenced by pollen exposure beyond the capability of current methods.
Int Arch Allergy Immunol

中文翻译：

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可以使用肽-噬菌体展示来改进针对PR-10-特定表位的抗体季节性变化的鉴定。

背景：在花粉症患者中，过敏原特异性抗体滴度显示季节性变化。对于这些抗原决定簇水平的变化知之甚少。目的：我们旨在利用肽噬菌体展示方法调查与Bet v 1相关食品过敏的患者的过敏原表位识别水平的季节性变化。方法：包括从桦树相关的大豆过敏免疫治疗试验的4名安慰剂组中收集的超过1年的血清样本。为了从Bet v 1相关食物过敏原中识别表位，将患者血清用于肽噬菌体展示实验。在计算机分析中进行了与变应原相关的丰富基序的分析。结果：我们确定了与Bet v 1及其在大豆和榛子中的同源物（分别为Gly m 4和Cor a 1）有关的表位基序，这些基序根据桦木和榛子花粉的暴露而富集。在桦树花粉季节达到峰值后的几周内，患者之间识别出的表位基序的模式差异很大。为一名被调查患者确定的同源Bet v 1和Cor a 1表位基序中氨基酸偏爱的数据表明，血清抗体针对Cor a 1表位基序的浓度或特异性发生了变化。结论：肽噬菌体展示数据表明桦木和榛子花粉暴露对Bet v 1样过敏原表位的识别模式有影响。面向表位的分析可以提供有关受花粉暴露影响的过敏原表位识别的更深入，个性化的详细信息，这超出了当前方法的能力。
Int Arch过敏免疫