Follicle culture in vitro provides a method for investigating stages of folliculogenesis that can lead to preserving fertility through cryopreservation techniques. This study aims to assess the effects of various concentrations of human follicular fluid (hFF) on growth, development, and expression of the proliferating cell nuclear antigen (PCNA) gene in mouse ovarian follicles in vitro. Preantral follicles were isolated from 14-day NMRI mouse ovaries. The follicles were cultured in basic media enriched with FBS, FSH, and insulin-transferrin-selenium, and supplemented with different concentrations of hFF (10, 20, and 30%) for 12 days. During the culture period, survival rate and follicular maturation, follicular diameter, levels of estrogen and progesterone secretion, and PCNA gene expression rate were evaluated. Survival rate, maturation, and antrum formation were significantly higher in the 10% hFF group than in the 20 and 30% hFF groups. On day 4, follicle diameter in the 10% hFF group was also higher than in the 20 and the 30% hFF group. In comparison with other groups, significantly higher estrogen and progesterone production levels were measured in the 10% hFF group. PCNA gene expression was also higher with 10 than 20 and 30% hFF concentrations. The present study suggests that addition of 10% hFF to mice ovarian preantral follicle culture media enhances follicle growth and oocyte maturation.

中文翻译：

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评估不同浓度的人卵泡液对小鼠卵巢卵泡生长、发育和 PCNA 基因表达的影响

体外卵泡培养提供了一种研究卵泡发生阶段的方法，可以通过冷冻保存技术保持生育能力。本研究旨在评估不同浓度的人卵泡液 (hFF) 对体外小鼠卵泡中增殖细胞核抗原 (PCNA) 基因的生长、发育和表达的影响。从 14 天的 NMRI 小鼠卵巢中分离出窦前卵泡。卵泡在富含 FBS、FSH 和胰岛素-转铁蛋白-硒的基础培养基中培养，并补充不同浓度的 hFF（10%、20% 和 30%），持续 12 天。在培养期间，评估存活率和卵泡成熟度、卵泡直径、雌激素和孕激素分泌水平以及PCNA基因表达率。存活率、成熟度、10% hFF 组的胃窦形成率显着高于 20% 和 30% hFF 组。在第 4 天，10% hFF 组的卵泡直径也高于 20% 和 30% hFF 组。与其他组相比，在 10% hFF 组中测得的雌激素和孕激素水平明显更高。PCNA 基因表达也高于 10% 和 30% hFF 浓度。本研究表明，向小鼠卵巢窦前卵泡培养基中添加 10% hFF 可促进卵泡生长和卵母细胞成熟。在 10% hFF 组中测得的雌激素和孕激素产生水平显着更高。PCNA 基因表达也高于 10% 和 30% hFF 浓度。本研究表明，向小鼠卵巢窦前卵泡培养基中添加 10% hFF 可促进卵泡生长和卵母细胞成熟。在 10% hFF 组中测得的雌激素和孕激素产生水平显着更高。PCNA 基因表达也高于 10% 和 30% hFF 浓度。本研究表明，向小鼠卵巢窦前卵泡培养基中添加 10% hFF 可促进卵泡生长和卵母细胞成熟。