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Regulation of the Morphological and Physical Properties of a Soft Tissue Scaffold by Manipulating DD and DS of O-Carboxymethyl Chitin
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-08-26 , DOI: 10.1021/acsabm.0c00730 Liqing Zhao 1 , Wenjing Deng 1 , Muhammad Shahid Riaz Rajoka 1 , Dejiao Cai 1 , Tao Xing 1 , Yiguang Wu 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-08-26 , DOI: 10.1021/acsabm.0c00730 Liqing Zhao 1 , Wenjing Deng 1 , Muhammad Shahid Riaz Rajoka 1 , Dejiao Cai 1 , Tao Xing 1 , Yiguang Wu 1
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To improve the biocompatibility/biodegradability as well as to lower the cost of the popular glycosaminoglycan/collagen scaffold, a monocomponent’s polysaccharide scaffold based on biomimetic chemical modification of chitin from lower organisms was developed creatively. O-Carboxymethyl chitin (O-CMCH) was prepared by chloroacetic acid substitution of alkalized chitin. The cross-linked O-CMCH soft tissue scaffold was constructed by a sol–gel freeze-drying method. The key parameters of the O-CMCH molecular structure, the degree of deacetylation (DD), and the degree of substitution (DS) were used to regulate the morphology and physical properties of the scaffold. The optimized scaffolds were implanted subcutaneously in mice, and the inflammation reaction of surrounding tissues, dermal tissue growth, and scaffold degradation were observed dynamically by light microscopy and scanning electron microscopy. The results showed that the micropores of the scaffold constructed by O-CMCH with DD = 0.53 and DS = 0.61 were uniformly distributed and in communication with each other, and the pore size was 100–150 μm, with high porosity (93.52 ± 4.68%), high swelling ratio (1402 ± 70%), and high skeleton cross-linking degree (93.4 ± 4.6%). Its tensile strength reached 0.183 ± 0.009 MPa, and its elongation at break was 18.7 ± 0.9%. Furthermore, it could be degraded to less than 10% after 16 days in phosphate buffer solution (pH = 7.4) with 0.2 mg/mL lysozymes (≥ 20 000 U/mg). The early inflammation after implanting the optimized scaffolds in mice showed no difference compared with the control. The scaffold material induced dermal tissues to grow over it and was degraded gradually in vivo. The optimized scaffold regulated by DD and DS of O-CMCH possessed suitable morphology and physical properties for soft tissue engineering technology and exhibited a high applicable value.
中文翻译：
通过操纵 O-羧甲基甲壳素的 DD 和 DS 来调节软组织支架的形态和物理特性
为提高流行的糖胺聚糖/胶原蛋白支架的生物相容性/生物降解性，降低成本，创造性地开发了一种基于低等生物几丁质仿生化学修饰的单组分多糖支架。O-羧甲基甲壳素（O -CMCH）是通过氯乙酸取代碱化甲壳素制备的。交联O - CMCH软组织支架采用溶胶-凝胶冷冻干燥法构建。O的关键参数-CMCH分子结构、脱乙酰度（DD）和取代度（DS）用于调节支架的形态和物理性质。将优化后的支架植入小鼠皮下，通过光学显微镜和扫描电镜动态观察周围组织的炎症反应、真皮组织的生长和支架的降解情况。结果表明，O构建的支架微孔-CMCH DD = 0.53 和 DS = 0.61 分布均匀，相互连通，孔径为100-150 μm，孔隙率高（93.52±4.68%），溶胀率高（1402±70%），骨架交联度高（93.4±4.6%）。其抗拉强度达到0.183±0.009 MPa，断裂伸长率为18.7±0.9%。此外，在含有 0.2 mg/mL 溶菌酶（≥ 20 000 U/mg）的磷酸盐缓冲溶液（pH = 7.4）中 16 天后，它可以降解到 10% 以下。将优化后的支架植入小鼠体内后的早期炎症与对照组相比没有差异。支架材料诱导真皮组织在其上生长并在体内逐渐降解。O的DD和DS调控的优化支架-CMCH具有适合软组织工程技术的形态和物理性质，具有较高的应用价值。
更新日期：2020-09-21
中文翻译：
通过操纵 O-羧甲基甲壳素的 DD 和 DS 来调节软组织支架的形态和物理特性
为提高流行的糖胺聚糖/胶原蛋白支架的生物相容性/生物降解性，降低成本，创造性地开发了一种基于低等生物几丁质仿生化学修饰的单组分多糖支架。O-羧甲基甲壳素（O -CMCH）是通过氯乙酸取代碱化甲壳素制备的。交联O - CMCH软组织支架采用溶胶-凝胶冷冻干燥法构建。O的关键参数-CMCH分子结构、脱乙酰度（DD）和取代度（DS）用于调节支架的形态和物理性质。将优化后的支架植入小鼠皮下，通过光学显微镜和扫描电镜动态观察周围组织的炎症反应、真皮组织的生长和支架的降解情况。结果表明，O构建的支架微孔-CMCH DD = 0.53 和 DS = 0.61 分布均匀，相互连通，孔径为100-150 μm，孔隙率高（93.52±4.68%），溶胀率高（1402±70%），骨架交联度高（93.4±4.6%）。其抗拉强度达到0.183±0.009 MPa，断裂伸长率为18.7±0.9%。此外，在含有 0.2 mg/mL 溶菌酶（≥ 20 000 U/mg）的磷酸盐缓冲溶液（pH = 7.4）中 16 天后，它可以降解到 10% 以下。将优化后的支架植入小鼠体内后的早期炎症与对照组相比没有差异。支架材料诱导真皮组织在其上生长并在体内逐渐降解。O的DD和DS调控的优化支架-CMCH具有适合软组织工程技术的形态和物理性质，具有较高的应用价值。
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