Abstract

Pollution is caused because of poor disposal of solid and liquid waste from the leather industry and release 20% of treated effluent and while the rest of 80% is untreated. The tannery effluent is a house for many antibiotic resistant bacteria and multi-drug resistant bacteria and these bacteria cause various bacterial infections which cannot be treated by antibiotics. This current work is focused on isolation and identification of antibiotic resistant strains from tannery effluent. To eliminate the antibiotic resistant strains from tannery effluent and make it beneficial to the society, green nanocolloid was synthesized from traditional medicinal plant Andographis paniculata. Various physio-chemical characterizations such as UV-Vis spectrum, FT-IR spectrum, Particle size analysis, Zeta potential analysis, FE-SEM and elemental analysis using EDAX were employed to identify the characteristic nature of green silver nanocolloids. Antibacterial and antibiofilm assays were performed to validate the efficacy of green nanocolloids. The results showed that ApNc had potent Antibacterial and antibio-film activity against antibiotic resistant strains isolated from tannery effluent. These green nanocolloids could be the alternate source to eradicate antibiotic resistant strains thriving in the tannery effluent. In future this formulation can be employed as a nanospary to avoid the spread of antibiotic resistant strains entering into human and animal system. This research work has been undertaken for the benefit of the society to provide people with better resources and to establish an environment that is disease free and eco-friendly.

摘要

造成污染的原因是皮革工业对固体和液体废物的处理不善，并释放了20％的经处理废水，而其余80％未经处理。制革厂废水中存有许多抗生素抗性细菌和多药抗性细菌，这些细菌会引起各种细菌感染，无法用抗生素治疗。目前的工作集中在从制革厂废水中分离和鉴定抗生素抗性菌株。为了消除制革厂废水中的抗生素耐药菌株并使其对社会有益，从传统药用植物Andographis paniculata合成了绿色纳米胶体。各种理化特性，如紫外-可见光谱，傅立叶变换红外光谱，粒度分析，Zeta电位分析，FE-SEM和使用EDAX的元素分析，均用于鉴定绿色银纳米胶体的特征。进行了抗菌和生物膜测定，以验证绿色纳米胶体的功效。结果表明，ApNc对制革厂废水中分离出的抗生素抗性菌株具有有效的抗菌和抗生物膜活性。这些绿色纳米胶体可能是根除制革厂废水中兴旺的抗生素抗性菌株的替代来源。将来，该制剂可以用作纳米喷雾剂，以避免抗生素抗性菌株扩散到人和动物系统中。