Abstract Phosphorous (P) that upholds life become unattainable as most of them become unavailable due to the formation of insoluble complexes with cations such as Ca2+, Al3+ and Fe3+ forming a complex calcium phosphate (Ca3(PO4)2), aluminum phosphate (AlPO) and ferrous phosphate (FePO) that results in the decrease of soluble P to a greater extent. There are several reports stating that several rhizospheric fungal species play an important role in solubilizing these insoluble phosphates into a soluble form by the excretion of enzymes like phosphatase, phytase enzymes, and organic acids. In view of this, so we have collected twenty fungal isolates having probable phosphate solubilizing efficiency from different regions of Lucknow, India. Their morphological and biochemical characteristics were tested. Among all, six efficient fungal isolates were further checked at molecular level by using 18S rRNA universal primers and by RAPD means. A dendrogram indicated 40-90% homology i.e., highest similarity was found in between species of Aspergillus flavus and A. biplanus with 33.8% similarity while minimum similarity was observed among A. flavus and Fusarium oxysporum. These findings suggest RAPD proves as, a reliable molecular tool that helps in strain specific discrimination.

中文翻译：

---

参与磷酸盐增溶的真菌群落多样性的分子检测和探索

摘要 维持生命的磷 (P) 变得难以获得，因为与 Ca2+、Al3+ 和 Fe3+ 等阳离子形成不溶性复合物，形成复合磷酸钙 (Ca3(PO4)2)、磷酸铝 (AlPO)和磷酸亚铁 (FePO) 导致可溶性 P 减少到更大程度。有几份报告指出，几种根际真菌通过分泌磷酸酶、植酸酶和有机酸等酶，在将这些不溶性磷酸盐溶解成可溶形式方面发挥重要作用。有鉴于此，我们从印度勒克瑙的不同地区收集了 20 株可能具有磷酸盐溶解效率的真菌分离株。测试了它们的形态和生化特性。所有之中，使用18S rRNA通用引物和RAPD方法在分子水平上进一步检查了6个有效的真菌分离物。树状图表明40-90%的同源性，即在黄曲霉和双平面曲霉的物种之间发现最高的相似性，具有33.8%的相似性，而在黄曲霉和尖孢镰刀菌之间观察到最小的相似性。这些发现表明 RAPD 被证明是一种可靠的分子工具，有助于区分菌株。