Abstract

Fibrinolytic enzymes have been considered promising for treatment and protection of healthy circulation due its ability to dissolve the fibrin in blood clots. Extractive fermentation is a not explored and efficient downstream process which segregates the desired product simultaneously in a fermentation process fast and economically. Extraction of fibrinolytic enzymes by Bacillus stearothermophilus DPUA 1729 employing conventional aqueous two-phase systems (ATPS) and extractive fermentation with ATPS was evaluated. The results of both systems were compared using a factorial design with PEG molar mass, PEG and salt concentrations as independent variables and extraction parameters as a response. In all conditions evaluated it was observed a similar partitioning of fibrinolytic enzymes through the phases, both in conventional ATPS and extractive fermentation. Salt concentration and interaction among PEG and salt concentration influenced in the partition coefficient. The fibrinolytic activity was determined by hydrolysis of fibrin in plate using the extract of one condition from extractive fermentation. The zone degradation presented a diameter of 7.03 ± 0.94 mm. In conclusion, there was no significant difference among the results obtained using conventional ATPS and extractive fermentation, however, the second one presents more advantages and can integrate production and extraction in one single step, reducing the costs.

抽象的

由于纤溶酶具有溶解血栓中血纤蛋白的能力，因此被认为有望用于治疗和保护健康的血液循环。提取发酵是一种尚未探索和有效的下游工艺，它可以快速，经济地在发酵过程中同时分离出所需的产物。嗜热脂肪芽孢杆菌提取纤溶酶使用常规水相两相系统（ATPS）并使用ATPS进行提取发酵的DPUA 1729进行了评估。使用因子设计比较两个系统的结果，其中PEG摩尔质量，PEG和盐浓度作为自变量，提取参数作为响应。在所评估的所有条件下，在常规ATPS和萃取发酵中均观察到纤溶酶在各相中的分配相似。盐浓度以及PEG之间的相互作用和盐浓度影响分配系数。通过使用一种来自提取发酵的条件的提取物，通过平板中纤维蛋白的水解来确定纤维蛋白溶解活性。区域退化的直径为7.03±0.94毫米。综上所述，