ABSTRACT Dietary capsaicin exhibits anti-steatosis activity in obese mice. High-fat diet (HFD)-induced mice is a highly studied approach to develop non-alcoholic fatty liver disease (NAFLD). In this study, we determined whether the topical application of capsaicin can improve lesions of NAFLD. The HFD-induced mice were treated with daily topical application of capsaicin for 8 weeks. Topical application of capsaicin reduced liver fat in HFD-fed mice. Capsaicin stimulated carnitine palmitoyl transferase (CPT)-1 and CD36 expression, which are associated with β-oxidation and fatty acids influx of liver while it decreased the expression of key enzymes involved in the synthesis of fatty acids, such as acetyl Co-A carboxylase (ACC) and fatty acid synthase (FAS). Immunohistochemical analysis revealed the elevated level of adiponectin in liver tissue of the capsaicin-treated mice. These results suggest that the topical application of capsaicin suppresses liver fat accumulation through the upregulation of β-oxidation and de novo lipogenesis in HFD-induced NAFLD mice.

中文翻译：

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辣椒素抑制高脂饮食诱导的 NAFLD 小鼠的肝脏脂肪积累

摘要膳食辣椒素在肥胖小鼠中具有抗脂肪变性活性。高脂肪饮食 (HFD) 诱导的小鼠是发展非酒精性脂肪肝 (NAFLD) 的一种经过深入研究的方法。在这项研究中，我们确定了辣椒素的局部应用是否可以改善 NAFLD 的病变。HFD 诱导的小鼠每天局部应用辣椒素治疗 8 周。辣椒素的局部应用减少了 HFD 喂养小鼠的肝脏脂肪。辣椒素刺激肉碱棕榈酰转移酶 (CPT)-1 和 CD36 表达，这与 β-氧化和脂肪酸流入肝脏有关，同时降低参与脂肪酸合成的关键酶的表达，如乙酰辅酶 A 羧化酶(ACC) 和脂肪酸合成酶 (FAS)。免疫组织化学分析显示，辣椒素处理的小鼠肝组织中脂联素水平升高。这些结果表明，辣椒素的局部应用通过在 HFD 诱导的 NAFLD 小鼠中上调β-氧化和从头脂肪生成来抑制肝脏脂肪积累。