Abstract This study describes an ex vivo model that creates an environment for dermatophyte biofilm growth, with features that resemble those of in vivo conditions, designing a new panorama for the study of antifungal susceptibility. Regarding planktonic susceptibility, MIC ranges were 0.125-1 µg ml−1 for griseofulvin and 0.000097-0.25 µg ml−1 for itraconazole and terbinafine. sMIC50 ranges were 2->512 µg ml−1 for griseofulvin and 0.25->64 µg ml−1 for itraconazole and terbinafine. CLSM images demonstrated a reduction in the amount of cells within the biofilm, but hyphae and conidia were still observed and biofilm biomass was maintained. SEM analysis demonstrated a retraction in the biofilm matrix, but fungal structures and water channels were preserved. These results show that ex vivo biofilms are more tolerant to antifungal drugs than in vitro biofilms, suggesting that environmental and nutritional conditions created by this ex vivo model favor biofilm growth and robustness, and hence drug tolerance.

中文翻译：

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皮肤癣菌的体外和体外生物膜：抗真菌药物研究的新全景

摘要 本研究描述了一种离体模型，该模型为皮肤癣菌生物膜的生长创造了一个环境，其特征类似于体内条件，为抗真菌敏感性研究设计了一个新的全景图。关于浮游生物敏感性，灰黄霉素的 MIC 范围为 0.125-1 µg ml-1，伊曲康唑和特比萘芬的 MIC 范围为 0.000097-0.25 µg ml-1。sMIC50 范围是 2->512 µg ml-1 灰黄霉素和 0.25->64 µg ml-1 伊曲康唑和特比萘芬。CLSM 图像表明生物膜内细胞数量减少，但仍观察到菌丝和分生孢子，生物膜生物量得以维持。SEM 分析表明生物膜基质收缩，但保留了真菌结构和水道。