Extracellular polymeric substance‐mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment,Plasma Processes and Polymers

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Extracellular polymeric substance‐mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment
Plasma Processes and Polymers ( IF 2.9 ) Pub Date : 2020-08-25 , DOI: 10.1002/ppap.202000108
Nid'a H. Alshraiedeh _{1,

2} , Stephen A. Kelly ₂ , Thomas P. Thompson _{2,

3} , Padrig B. Flynn ₂ , Michael M. Tunney ₂ , Brendan F. Gilmore ₂

Affiliation

Pseudomonas aeruginosa is an opportunistic disease‐causing bacterium, with a number of strains exhibiting a mucus‐forming (mucoid) phenotype during infection, producing biofilms with a surrounding matrix containing alginate. Atmospheric pressure nonthermal plasmas (APNTP) are an emerging, potential approach to control biofilms across a range of medical and industrial applications. In this study, we examine the effect of plasma treatment on P. aeruginosa biofilms from clinical samples of cystic fibrosis patients, exhibiting both mucoid and non‐mucoid types. Biofilms of mucoid strains exhibit significantly elevated APNTP tolerance (p < .05). Endogenous alginate overproduction, as well as supplementation of P. aeruginosa cultures with exogenous alginate, results in significantly increased APNTP tolerance. Overall, this study shows how extracellular polymeric substance components mediate tolerance to APNTP, with significantly greater effects observed in mucoid strains.

中文翻译：

胞外聚合物介导的铜绿假单胞菌生物膜对大气压非热等离子体处理的耐受性

铜绿假单胞菌是一种机会性致病细菌，许多菌株在感染过程中表现出粘液形成（粘液样）表型，产生具有周围基质的藻酸盐生物膜。大气压非热等离子体（APNTP）是一种新兴的潜在方法，可用于控制一系列医学和工业应用中的生物膜。在这项研究中，我们检查了血浆处理对囊性纤维化患者临床样本中铜绿假单胞菌生物膜的影响，表现出粘液性和非粘液性两种类型。粘液样菌株的生物膜表现出显着提高的APNTP耐受性（p <.05）。内源性藻酸盐过度生产以及铜绿假单胞菌的补充外源海藻酸盐培养可显着提高APNTP耐受性。总的来说，这项研究表明细胞外聚合物质成分如何介导对APNTP的耐受性，在粘液状菌株中观察到明显更大的影响。

更新日期：2020-08-25

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