BACKGROUND Lignocellulosic wastes are pretreated prior to their utilization in fermentation processes. Since pretreatment also alters the topological features of the substrates, therefore, the suitability of pretreated waste as immobilization matrix for microbial cells needs investigation. RESULTS In this study, the effect of chemical pretreatment to sugarcane bagasse (SB) for its subsequent utilization as a matrix to immobilize a pectinolytic yeast, Geotrichum candidum AA15, was evaluated by cell retention, concentration of immobilized cells, immobilization efficiency, scanning electron micrographs and Fourier transform infrared spectroscopy of the substrate and pectinase titers obtained after recycling. The results revealed that untreated SB is more efficient for immobilization with the higher values of cell retention and pectinase productivity (99.78%) that retained for up to 6 production cycle. It was deduced that removal of lignin by pretreatment negatively influenced the ability of SB to support cells adhesion as lignin acts as a sealing agent that provides strength to the substrate. CONCLUSION The strategy to utilize SB as immobilization matrix was found effective at lab scale as it improved the pectinase production and may be investigated further for its large scale and cost-effective production. This article is protected by copyright. All rights reserved.

中文翻译：

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木质素在甘蔗渣上酵母固定化连续果胶酶生产中的支持作用

背景木质纤维素废物在它们用于发酵过程之前被预处理。由于预处理也会改变底物的拓扑特征，因此，预处理废物作为微生物细胞固定基质的适用性需要研究。结果在本研究中，化学预处理对甘蔗渣 (SB) 的影响随后用作基质固定果胶酵母，Getrichum candidum AA15，通过细胞保留、固定细胞浓度、固定效率、扫描电子显微照片进行评估回收后得到的底物和果胶酶滴度的傅里叶变换红外光谱。结果表明，未经处理的 SB 固定化效率更高，细胞保留值和果胶酶生产力 (99.78%) 值更高，保留时间长达 6 个生产周期。据推断，通过预处理去除木质素会对 SB 支持细胞粘附的能力产生负面影响，因为木质素充当为基材提供强度的密封剂。结论 发现利用 SB 作为固定基质的策略在实验室规模是有效的，因为它提高了果胶酶的生产，并且可以进一步研究其大规模和具有成本效益的生产。本文受版权保护。版权所有。据推断，通过预处理去除木质素会对 SB 支持细胞粘附的能力产生负面影响，因为木质素充当为基材提供强度的密封剂。结论 发现利用 SB 作为固定基质的策略在实验室规模是有效的，因为它提高了果胶酶的生产，并且可以进一步研究其大规模和具有成本效益的生产。本文受版权保护。版权所有。据推断，通过预处理去除木质素会对 SB 支持细胞粘附的能力产生负面影响，因为木质素充当为基材提供强度的密封剂。结论 发现利用 SB 作为固定基质的策略在实验室规模是有效的，因为它提高了果胶酶的生产，并且可以进一步研究其大规模和具有成本效益的生产。本文受版权保护。版权所有。