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Molecular sex identification of Malaysian White‐Nest Swiftlet (Aerodramus fuciphagus Thunberg, 1812)
Ecology and Evolution ( IF 2.3 ) Pub Date : 2020-08-25 , DOI: 10.1002/ece3.6699
Muhammad Amin Osman 1 , Sumita Sugnaseelan 1 , Jothi Malar Panandam 2 , Nurul Izza Ab Ghani 3
Affiliation  

The difficulty in differentiating the sex of monomorphic bird species has made molecular sexing an important tool in addressing this problem. This method uses noninvasively collected materials such as feathers and may be advantageous for sexing endangered as well as commercialized bird species. In this study, seven primer sets for sexing birds were screened in Aerodramus fuciphagus using a total of 13 feather samples that were randomly selected from the state of Perak, Malaysia. From the screening analysis, only one primer set (P8/WZ/W) successfully differentiated the sex of A. fuciphagus. PCR amplification produced a single 255‐bp DNA fragment for males which was derived from CHD‐Z (CHD gene region in the sex chromosome Z), while for the females it produced two fragments (144 and 255 bp). The 144‐bp fragment was from CHD‐W (CHD gene region in the sex chromosome W). Results from sequencing showed no variations in the base sequences of the CHD‐W and CHD‐Z amplified fragments within the same sexes, except for one male sample (A23) where at position 166, a base substitution occurred (G → A). Phylogenetic analysis of CHD‐W showed that four (Apodiformes; Gruiformes; Passeriformes; and Pelecaniformes) out of the five orders investigated had formed four clear clusters within their orders, including the studied order: Apodiformes. Whereas in CHD‐Z, four (Accipitriformes; Columbiformes; Galliformes; and Passeriformes) out of five orders investigated formed four clear clusters within their orders, excluding the studied order. In addition, A. fuciphagus and Apus apus (both Apodiformes) showed less divergence in CHD‐W than CHD‐Z (0% c.f. 9%). The result suggests that in A. fuciphagus, CHD gene evolution occurred at a higher rate in males (CHD‐Z) compared to females (CHD‐W). This finding may be useful for further studies on sex ratio and breeding management of A. fuciphagus.

中文翻译:


马来西亚白巢金丝燕 (Aerodramus fuciphagus Thunberg, 1812) 的分子性别鉴定



区分单一形态鸟类性别的困难使得分子性别鉴定成为解决这一问题的重要工具。这种方法使用非侵入性收集的材料,例如羽毛,可能有利于濒危鸟类和商业化鸟类的性别鉴定。在这项研究中,使用从马来西亚霹雳州随机挑选的总共 13 个羽毛样本,在Aerodramus fuciphagus中筛选了 7 个用于鸟类性别鉴定的引物组。从筛选分析来看,只有一套引物(P8/WZ/W)成功区分了褐藻的性别。 PCR 扩增产生了男性的单个 255 bp DNA 片段,该片段源自CHD-Z (性染色体 Z 中的 CHD 基因区域),而女性则产生了两个片段(144 和 255 bp)。 144 bp 片段来自CHD-W (性染色体 W 中的 CHD 基因区域)。测序结果显示,同一性别的CHD-WCHD-Z扩增片段的碱基序列没有变化,除了一个男性样本 (A23) 在第 166 位发生了碱基替换 (G → A)。 CHD-W的系统发育分析表明,所调查的 5 个目中的 4 个目(伞形目、鹤形目、雀形目和鹈鹕目)在其目内形成了四个清晰的簇,包括所研究的目:伞形目。而在CHD-Z中,调查的 5 个目中有 4 个(鹰形目、鸽形目、鸡形目和雀形目)在其目内形成了四个清晰的簇,不包括所研究的目。此外, A. fuciphagus 和Apus apus (均为 Apodiformes)在CHD-W中表现出的差异小于CHD-Z (0% cf . 9%)。结果表明,在A. fuciphagus中,雄性 ( CHD-Z ) 的 CHD 基因进化发生率高于雌性 ( CHD-W )。这一发现可能对进一步研究褐藻的性别比和繁殖管理具有一定的参考价值。
更新日期:2020-10-12
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