Substrate utilization strategies of microorganisms modulate soil organic carbon (SOC) turnover, which is essentially regulated by the quality and quantity of exogenous substrate. However, a detailed understanding of the functional traits of different microbial taxa in substrate utilization is limited. Therefore, an 8-week microcosm amendment of the weekly addition of ¹³C-glucose was performed on an Ultisol to link the phylogenetic classification of microbial taxa with their activation via the use of exogenous labile carbon (C). Temporal variations in glucose-C assimilation of bacterial and fungal groups were characterized using the combined application of nucleic acid stable isotope probing and high-throughput pyrosequencing techniques. The stimulation of glucose on the growth of soil microorganisms was time-dependent, with a rapid response of bacteria and a succession to fungal proliferation. The preference of C sources of bacterial phyla was definitely controlled by the life-history of trophic pattern, and the copiotrophic bacterial phyla remained in stronger competitiveness for labile C compared to the oligotrophic phyla. These substrate utilization strategies primarily originated from the genus level with phylogenetically conserved attributes despite the increased number of functional executors with the increasing C availability. In contrast, all of the detected fungi in the incubation microcosm used labile C and possible the native soil organic matter from the phylum to genus level, which was primarily ascribed to the wide spectrum of substrate utilization of fungal taxa. The distinct substrate utilization strategies of bacteria and fungi have important implications in the elucidation of their functional traits involved in SOC turnover.

微生物的底物利用策略可调节土壤有机碳（SOC）的转化，这主要受外源底物的质量和数量的调节。但是，对不同微生物分类群在底物利用中的功能特征的详细了解是有限的。因此，每周增加¹³个为期8周的缩影C-葡萄糖在Ultisol上进行，通过使用外源不稳定碳（C）将微生物分类群的系统发育分类与其激活联系起来。细菌和真菌基团的葡萄糖-C同化作用的时间变化是通过结合使用核酸稳定同位素探测和高通量焦磷酸测序技术来表征的。葡萄糖对土壤微生物生长的刺激是时间依赖性的，具有细菌的快速反应和对真菌增殖的连续作用。细菌门的C来源的偏好绝对受营养模式的生活史控制，与营养丰富的门相比，营养营养的细菌门对不稳定的C仍然具有较强的竞争力。这些底物利用策略主要源自具有系统进化上保守属性的属水平，尽管功能执行者的数量随着碳可用性的增加而增加。相比之下，在孵化微观世界中所有检测到的真菌都使用了不稳定的C，并可能使用了从菌门到属水平的天然土壤有机物，这主要归因于真菌类群的广泛底物利用。细菌和真菌的独特底物利用策略对阐明涉及SOC转​​换的功能性状具有重要意义。孵育微观世界中所有检测到的真菌都使用了不稳定的C，并且可能从门到属水平都使用了天然土壤有机物，这主要归因于真菌类群的广泛底物利用。细菌和真菌的独特底物利用策略对阐明涉及SOC转​​换的功能性状具有重要意义。孵育微观世界中所有检测到的真菌都使用了不稳定的C，并且可能从门到属水平都使用了天然土壤有机物，这主要归因于真菌类群的广泛底物利用。细菌和真菌的独特底物利用策略对阐明SOC转换所涉及的功能特性具有重要意义。