Hair regenerative medicine is a promising approach for the treatment of hair loss and involves the transplantation of follicular stem cells into bald spots to regenerate hair. Various approaches have been investigated to engineer tissue grafts for use in hair regenerative medicine. Tissue-like three-dimensional aggregates, such as bioengineered hair follicle germs (HFGs), have shown great promise for hair regeneration, with normal tissue morphology and hair cycles. However, these approaches have not yet been applied in clinical settings, and further studies are needed to improve hair generation efficiency. The biological molecules in in vivo microenvironments around HFGs may provide cues for the in vitro preparation of HFGs with higher trichogenic functionalities. Activated platelet-rich plasma releasate (PRPr) is an autologous source of signaling molecules including growth factors and cytokines. In this study, we investigated the effects of PRPr on the preparation of HFGs in vitro. The presence of PRPr did not hinder the spontaneous formation of dumbbell-like HFGs from a suspension of embryonic skin-derived epithelial and mesenchymal cells in a custom-designed HFG culture plate. HFGs prepared with PRPr displayed greater levels of follicular gene expression compared to those prepared in the absence of PRPr. Moreover, the hair regeneration ability upon intracutaneous transplantation was significantly improved in the presence of PRPr. These results suggest that PRPr is beneficial for engineering HFGs for autologous hair regenerative medicine.

头发再生医学是治疗脱发的一种有前途的方法，涉及将滤泡干细胞移植到秃头部位以再生头发。已经研究了多种方法来工程设计用于头发再生医学的组织移植物。具有组织结构的三维聚集体，例如生物工程化的毛囊胚芽（HFGs），具有正常的组织形态和毛发循环，对头发再生具有广阔的前景。但是，这些方法尚未在临床环境中应用，需要进一步的研究来提高头发的产生效率。HFGs体内微环境中的生物分子可能为体外提供线索制备具有更高生摩擦功能的HFG。活化的富含血小板的血浆释放物（PRPr）是包括生长因子和细胞因子在内的信号分子的自体来源。在这项研究中，我们调查了PRPr对HFGs体外制备的影响。在定制设计的HFG培养板中，PRPr的存在并不妨碍从胚胎皮肤衍生的上皮和间充质细胞的悬浮液自发形成哑铃状HFG。与不使用PRPr制备的HFG相比，使用PRPr制备的HFG表现出更高的卵泡基因表达水平。此外，在PRPr的存在下，皮内移植后的头发再生能力显着提高。这些结果表明PRPr对于工程化用于自发再生医学的HFG是有益的。