Transcription factors (TFs) as key regulators play crucial roles in biological processes. The identification of TF–target regulatory relationships is a key step for revealing functions of TFs and their regulations on gene expression. The accumulated data of chromatin immunoprecipitation sequencing (ChIP-seq) provide great opportunities to discover the TF–target regulations across different conditions. In this study, we constructed a database named hTFtarget, which integrated huge human TF target resources (7190 ChIP-seq samples of 659 TFs and high-confidence binding sites of 699 TFs) and epigenetic modification information to predict accurate TF–target regulations. hTFtarget offers the following functions for users to explore TF–target regulations: (1) browse or search general targets of a query TF across datasets; (2) browse TF–target regulations for a query TF in a specific dataset or tissue; (3) search potential TFs for a given target gene or non-coding RNA; (4) investigate co-association between TFs in cell lines; (5) explore potential co-regulations for given target genes or TFs; (6) predict candidate TF binding sites on given DNA sequences; (7) visualize ChIP-seq peaks for different TFs and conditions in a genome browser. hTFtarget provides a comprehensive, reliable and user-friendly resource for exploring human TF–target regulations, which will be very useful for a wide range of users in the TF and gene expression regulation community. hTFtarget is available at http://bioinfo.life.hust.edu.cn/hTFtarget.

转录因子（TFs）作为关键调控因子在生物过程中起着至关重要的作用。TF-靶标调控关系的鉴定是揭示TFs功能及其对基因表达调控的关键步骤。染色质免疫沉淀测序（ChIP-seq）的累积数据为发现不同条件下的TF-靶标调控提供了巨大的机会。在这项研究中，我们构建了一个名为hTFtarget的数据库，该数据库集成了庞大的人TF目标资源（659个TF的7190个ChIP-seq样本和699个TF的高可信度结合位点）和表观遗传修饰信息可预测准确的TF-目标法规。hTFtarget为用户提供了以下功能，以探索TF目标规则：（1）在数据集中浏览或搜索查询TF的一般目标；（2）浏览TF-目标法规以查询特定数据集或组织中的TF；（3）搜索给定目标基因或非编码RNA的潜在TF；（4）研究细胞系中TF之间的共缔合；（5）探索给定目标基因或TF的潜在共调控；（6）预测给定DNA序列上的候选TF结合位点；（7）在基因​​组浏览器中可视化不同TF和条件的ChIP-seq峰。hTFtarget提供了全面，可靠且人性化的资源，用于探索人类TF-Target法规，这对于TF和基因表达法规界的广大用户将非常有用。hTFtarget可从http://bioinfo.life.hust.edu.cn/hTFtarget获得。