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Bioactive polysaccharides and their soluble fraction from Tossa jute (Corchorus olitorius L.) leaves
Food Bioscience ( IF 4.8 ) Pub Date : 2020-08-26 , DOI: 10.1016/j.fbio.2020.100741
Amira Racha Ben Yakoub , Ola Abdehedi , Mourad Jridi , Walid Elfalleh , Intidhar Bkhairia , Moncef Nasri , Ali Ferchichi

The biological proprieties of mucilaginous polysaccharides (PSc) and their soluble fraction (SF) extracted from Tossa jute leaves (Corchorus olitorius L.) using an ethanol precipitation method were studied. The results showed that PSc had higher total polyphenols and flavonoids and greater antioxidant activities than SF. At 1.5 mg/ml PSc, the antioxidant activities were about 90% against 1,1-diphenyl-2-picryl hydrazyl radical (DPPH•), 78% against lipid peroxidation and 69% against β-carotene. The PSc with a FRAP assay at the same concentration showed an effective protection against hydroxyl radicals and DNA breakage. Furthermore, the extracted PSc had a wide spectrum of antibacterial activities against all bacteria tested (Gram+ and Gram-) while SF was effective against Klebsiella pneumoniae and Salmonella enterica at 25 mg/ml. The overall data suggested that this natural PSc may be used as a competitive antioxidant and antimicrobial additive in food and in medicinal preparations.



中文翻译:

生物活性多糖和托萨黄麻(其可溶部分长蒴黄麻L.)叶

研究了利用乙醇沉淀法从黄麻叶片(Corchorus olitorius L.)提取的粘多糖(PSc)及其可溶性级分(SF)的生物学特性。结果表明,PSc比SF具有更高的总多酚和类黄酮含量和更大的抗氧化活性。在1.5 mg / ml PSc下,抗氧化活性约为1,1-二苯基-2-吡啶基肼基(DPPH•),抗脂质过氧化反应的78%和抗β-胡萝卜素的抗氧化剂的78%。用相同浓度的FRAP测定的PSc对羟基自由基和DNA断裂显示出有效的保护作用。此外,提取的PSc对所有测试的细菌(Gram +和Gram-)具有广泛的抗菌活性,而SF对肺炎克雷伯菌有效沙门氏菌25 mg / ml。总体数据表明,这种天然PSc可用作食品和药物制剂中的竞争性抗氧化剂和抗菌添加剂。

更新日期:2020-08-26
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