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Microsatellite multiplex assay for sable ( Martes zibellina ) and pine marten ( Martes martes )
Mammal Research ( IF 1.3 ) Pub Date : 2020-08-25 , DOI: 10.1007/s13364-020-00529-4
Makar Modorov , Vladimir Monakhov , Vladimir Mikryukov , Nikolai Erokhin , Inga Tkachenko , Maria Polezhaeva , Maryana Ranyuk

We evaluate the applicability of the microsatellite multiplex assay of 11 short tandem repeat loci for individual identification of sable and pine marten, taxonomic identification of Martes species, and assessment of inter-population separation of sable populations isolated by distance. Based on the screening, we detected 50 and 80–82 alleles in pine marten and two sable populations, respectively. All 11 microsatellite markers of the proposed PCR assay were characterized by the same range of allele length in the two Marten species, which makes species identification unfeasible using any of the loci alone. However, the combination of all 11 loci differentiated sables and pine martens, and more importantly provided the opportunity to identify their hybrids in the sympatry range zone. Using the proposed microsatellite assay, we successfully identified geographic origin of most genotyped sable individuals.

中文翻译:

貂(Martes zibellina)和松貂(Martes martes)的微卫星多重测定。

我们评估了11个短串联重复基因座的微卫星多元测定法的适用性,用于黑貂和松貂的个体鉴定,Martes物种的分类学鉴定以及按距离分离的黑貂种群的种群间分离。基于筛选,我们分别在松貂和两个黑貂种群中检测到50和80-82个等位基因。拟议的PCR分析的所有11个微卫星标记的特征是两个Marten中相同的等位基因长度范围物种,这使得单独使用任何一个基因座进行物种鉴定都不可行。但是,所有11个位点分化的黑貂和松树貂的组合,更重要的是提供了在交配点范围区域中识别它们的杂种的机会。使用提出的微卫星测定法,我们成功鉴定了大多数基因型黑貂个体的地理起源。
更新日期:2020-08-25
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