Background

Previous investigations reported that the imbalance of intestinal microflora may be the initiation and promotion factor in the pathogenesis of inflammatory bowel disease such as ulcerative colitis (UC). Glucocorticoid is a very important class of regulatory molecules in the body. The response of different individuals to glucocorticoids can be divided into glucocorticoid sensitive, glucocorticoid resistance and glucocorticoid dependence.

Objective

We aimed to investigate the differences in intestinal microflora composition and related metabolic pathways in UC patients with these three different glucocorticoid response types.

Methods

The whole genomic DNA was extracted from fecal specimens. High-throughput sequencing technology was used to analyze the fecal 16S rRNA genome of UC patients with different glucocorticoid response types, and functional prediction was performed by PICRUSTs software.

Results

The results showed that the intestinal microflora of the three groups were mainly composed of Firmicutes, Proteobacteria and Bacteroidetes. Although the species abundance and diversity of intestinal microflora in UC patients differed little among the three groups, the composition of intestinal microflora showed significant heterogeneity, which directly led to differences in the function of intestinal microbiota of UC patients with different glucocorticoid responses. Furthermore, of the 240 pathways, “PANTO-PWY: phosphopantothenate biosynthesis I”, “COA-PWY-1: coenzyme A biosynthesis II (mammalian)” and “PWY-4242: pantothenate and coenzyme A biosynthesis III” were significantly different in the three groups.

Conclusions

These results indicate that UC patients with different glucocorticoids response types have different bacterial compositions and functions, which lays a foundation for further study of glucocorticoid resistance in UC patients.

中文翻译：

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不同糖皮质激素反应类型溃疡性结肠炎患者肠道菌群差异的高通量测序分析。

背景

既往研究报道，肠道菌群失衡可能是溃疡性结肠炎（UC）等炎症性肠病发病的启动和促进因素。糖皮质激素是体内非常重要的一类调节分子。不同个体对糖皮质激素的反应可分为糖皮质激素敏感、糖皮质激素抵抗和糖皮质激素依赖。

客观的

我们旨在研究具有这三种不同糖皮质激素反应类型的 UC 患者肠道菌群组成和相关代谢途径的差异。

方法

从粪便标本中提取整个基因组 DNA。采用高通量测序技术分析不同糖皮质激素反应类型UC患者粪便16S rRNA基因组，通过PICRUSTs软件进行功能预测。

结果

结果表明，三组肠道菌群主要由厚壁菌门、变形菌门和拟杆菌门组成。尽管三组UC患者肠道菌群的物种丰度和多样性差异不大，但肠道菌群组成呈现显着异质性，直接导致不同糖皮质激素反应的UC患者肠道菌群功能存在差异。此外，在 240 条途径中，“PANTO-PWY：磷酸泛酸生物合成 I”、“COA-PWY-1：辅酶 A 生物合成 II（哺乳动物）”和“PWY-4242：泛酸和辅酶 A 生物合成 III”在三组。

结论

这些结果表明，不同糖皮质激素反应类型的UC患者具有不同的细菌组成和功能，为进一步研究UC患者的糖皮质激素抵抗奠定了基础。