The phylogeny and systematics of cordycipitoid fungi have been extensively studied in the last two decades. However, systematic positions of some taxa in the family Cordycipitaceae have not yet been thoroughly resolved. In this study, a new phylogenetic framework of Cordycipitaceae is reconstructed using multigene (nrSSU, nrLSU, tef-1α, rpb1 and rpb2) sequence data with large-scale taxon sampling. In addition, ITS sequence data of species belonging to the Lecanicillium lineage in the family Cordycipitaceae are used to further determine their phylogenetic placements. Based on molecular phylogenetic data together with morphological evidence, two new genera (Flavocillium and Liangia), 16 new species and four new combinations are introduced. In the new genus Flavocillium, one new species F. bifurcatum and three new combinations previously described as Lecanicillium, namely F. acerosium, F. primulinium and F. subprimulinium, are proposed. The genus Liangia is built by the new species Lia. sinensis with Lecanicillium-like asexual morph, isolated from an entomopathogenic fungus Beauveria yunnanensis. Due to the absence of Paecilomyces hepiali, an economically and medically significant fungus, in the earlier phylogenetic analyses, its systematic position has been puzzling in both business and academic communities for a long time. Here, P. hepiali is recharacterized using the holotype material along with seven additional samples. It is assigned to the genus Samsoniella (Cordycipitaceae, Hypocreales) possessing Cordyceps-like sexual morph and Isaria-like asexual morph, and thus a new combination, namely S. hepiali is proposed. An additional nine new species in Samsoniella are described: S. alpina, S. antleroides, S. cardinalis, S. cristata, S. lanmaoa, S. kunmingensis, S. ramosa, S. tortricidae and S. yunnanensis. Four new species in Cordyceps are described: C. chaetoclavata, C. cocoonihabita, C. shuifuensis and C. subtenuipes. Simplicillium yunnanense, isolated from synnemata of Akanthomyces waltergamsii, is described as a new species.

在过去的二十年中，虫草类真菌的系统发育和系统学已被广泛研究。但是，虫草科中某些分类单元的系统位置尚未彻底解决。在这项研究中，使用大规模分类群采样，使用多基因（nr SSU，nr LSU，tef-1α，rpb1和rpb2）序列数据重建了冬虫夏草的一个新的系统发育框架。另外，属于冬虫夏草科的Lecanicillium谱系物种的ITS序列数据被用于进一步确定其系统发生位置。基于分子系统发育数据和形态学证据，两个新属（大戟属和凉果），引入了16个新物种和4个新组合。在新的属Flavocillium，一个新的物种F. bifurcatum先前被描述为与三个新的组合蜡蚧，即F. acerosium，F. primulinium和F. subprimulinium，提出。亮雅属是由新种利亚（Lia）建立的。分离自昆虫病原真菌云南白僵菌的一种具有Lecanicillium- like无性形态的中华绒螯蟹。由于不存在肝拟青霉作为一种具有经济和医学意义的真菌，在较早的系统发育分析中，其系统地位长期以来一直困扰着商业和学术界。在这里，使用全型材料和七个其他样品对肝假单胞菌进行了重新定性。它被分配给属Samsoniella（Cordycipitaceae，肉座菌目）具有虫草样性变形和Isaria-像无性变形，并因此新的组合，即S.蝙蝠蛾提出。还描述了沙门氏菌中的另外九个新物种：高山链球菌，硬皮链球菌，卡迪纳尔链球菌，crisata链球菌，lanmaoa链球菌，昆明链球菌，S. ramosa，S。tortricidae和S. yunnanensis。描述了冬虫夏草中的四个新种：C. chaetoclavata，C。cocoonihabita，C。shuifuensis和C. subtenuipes。从拟南芥（Akanthomyces waltergamsii）的结膜分离的云南单形菌被描述为一个新物种。