Owing to differentiation and self-renewal capacity, hematopoietic stem cells clasp potentiality to engender all blood cell types, leading to their immense competence to play a diverse role in therapeutic applications. Although these stem cells are the most investigated and exploited until now, further research is still essential to comprehend their nature, fate, and potential. Enhanced usage of hematopoietic stem cells in research and therapeutics intensified the requirement of expansion and differentiation of hematopoietic stem cells under in vitro conditions. Since these cells remain in senescence for a prolonged period before isolation, selection of appropriate growth medium along with supplements and culture conditions are crucial to initiate their cell division and to designate their destiny. The precise equilibrium between self-renewal and differentiation of stem cells sustained by exclusive medium along with special growth or differentiation factors is accountable for generating diverse cell lineages. Maintenance of hematopoietic stem and progenitor cell lines along with the advancement of research work generate an inexorable demand for production and commercialization of specialized stem cell culture media, with or without serum along with specific growth factors and supplements. Media commercialization for precise stem cell types, culturing and differentiation is a cost-effective developing field. Here in this review, we are assembling various types of hematopoietic stem cell self-renewal, expansion and differentiation media along with supplements and culture conditions, either developed and used by various scientists or are available commercially.

中文翻译：

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造血干细胞的培养，扩增和分化：对可变和可用培养基的见解。

由于具有分化能力和自我更新能力，造血干细胞具有产生所有血细胞类型的潜力，从而使其具有在治疗应用中发挥多种作用的巨大能力。尽管这些干细胞是迄今为止进行最多研究和利用的，但进一步的研究对于理解它们的性质，命运和潜力仍然至关重要。造血干细胞在研究和治疗中的更多使用增加了体外扩增和分化造血干细胞的需求条件。由于这些细胞在分离前会长期处于衰老状态，因此选择合适的生长培养基以及补品和培养条件对于启动细胞分裂和确定命运至关重要。排他性培养基与特殊的生长或分化因子共同维持的干细胞自我更新与分化之间的精确平衡，是产生多种细胞谱系的原因。造血干细胞和祖细胞系的维护以及研究工作的发展，对专门的干细胞培养基的生产和商业化提出了不可或缺的需求，这些培养基具有或不具有血清以及特定的生长因子和补品。媒体商业化，以精确的干细胞类型，培养和差异化是一个具有成本效益的发展领域。在本综述中，我们正在组装各种类型的造血干细胞自我更新，扩增和分化培养基，以及补充剂和培养条件，这些培养剂是由不同科学家开发和使用的，也可以通过商业途径获得。