Human pluripotent stem cells (hPSCs) hold great promise for future applications in drug discovery and cell therapies. hPSC culture protocols require specific substrates and medium supplements to support cell expansion and lineage specific differentiation. The animal origin of these substrates is a severe limitation when considering the translation of hPSC derivatives to the clinic and in vitro disease modeling. The present study evaluates the use of a human placenta- derived extracellular matrix (ECM) hydrogel, HuGentra^Ⓡ, to support tri-lineage differentiation of human induced pluripotent stem cells (hiPSCs). Lineage-specific embryoid bodies (EBs) were plated onto three separate matrices, and differentiation efficiency was evaluated based on morphology, protein, and gene expression. HuGentra was found to support the differentiation of hiPSCs to all three germ layers: ectodermal, mesodermal, and endodermal lineages. hiPSCs differentiated into neurons, cardiomyocytes, and hepatocytes on HuGentra had similar morphology, protein, and gene expression compared to differentiation on Matrigel or other cell preferred matrices. HuGentra can be considered as a suitable human substrate for hiPSC differentiation.

中文翻译：

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人胎盘来源的ECM支持人诱导的多能干细胞的三系分化。

人多能干细胞（hPSC）在药物发现和细胞疗法中的未来应用前景广阔。hPSC培养方案需要特定的底物和培养基补充剂，以支持细胞扩增和谱系特异性分化。当考虑将hPSC衍生物翻译至临床和体外疾病模型时，这些底物的动物来源受到严重限制。本研究评估了人类胎盘来源的细胞外基质（ECM）水凝胶HuGentra ^the的使用支持人诱导的多能干细胞（hiPSC）的三系分化。将谱系特异性胚状体（EB）铺在三个单独的基质上，并根据形态，蛋白质和基因表达评估分化效率。发现HuGentra支持hiPSC分化为所有三个胚层：外胚层，中胚层和内胚层谱系。与在Matrigel或其他细胞首选基质上分化相比，在HuGentra上分化为神经元，心肌细胞和肝细胞的hiPSC具有相似的形态，蛋白质和基因表达。HuGentra可以被认为是hiPSC分化的合适人类底物。