This study examines the osteogenic differentiation promotion effect of micro/nanostructures of raffia on human adipose-derived stem cells to confirm the potential application of plant-derived micro/nanotopographies in tissue regeneration. The results confirm that the nanorod array on the front surface and the honeycomb-like microstructure on the back surface of raffia can not only regulate the adhesion, spreading, and migration of stem cells but also promote the osteogenic differentiation of the stem cells at a subsequent stage of cell culture. The osteocalcin expressions by the 21-day cultured cells on the front and back surfaces of raffia were 55-fold and 36-fold higher compared to the expression on a tissue culture plate. This indicates that plant-derived micro/nanotopographies can significantly promote stem cell differentiation. Furthermore, a general strategy for the application of plant-derived materials to stem-cell differentiation and bone-tissue engineering is suggested.

中文翻译：

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植物来源的微/纳米结构对干细胞分化的地形调控。

这项研究检查了酒椰微/纳米结构对人脂肪干细胞的成骨分化促进作用，以证实植物微/纳米地形学在组织再生中的潜在应用。结果证实，酒椰纤维前表面的纳米棒阵列和酒椰纤维后表面的蜂窝状微观结构不仅可以调节干细胞的粘附，扩散和迁移，还可以促进干细胞的成骨分化。细胞培养的阶段。与在组织培养板上的表达相比，在酒椰前后表面上培养21天的细胞的骨钙素表达分别高55倍和36倍。这表明植物来源的显微/纳米地形学可以显着促进干细胞分化。此外，