Bats play a key role as reservoir hosts of many emerging viral diseases with zoonotic potential. However, little is known about the laboratory reference intervals (RIs) of bats, especially Egyptian fruit bats (Rousettus aegyptiacus). The aim of this study was to obtain haematological, biochemical and electrophoretic RIs from captive fruit bats. Blood was collected from 21 R. aegyptiacus (11 females and 10 males). Complete blood cell count was performed using an impedance cell counter followed by the morphologic analysis of blood smears. Clinical biochemistry was performed with an automated spectrophotometer and agarose gel electrophoresis was carried out with an automated instrument. Reference intervals were determined using the Reference Value Advisor V2.1, following the American Society for Veterinary Clinical Pathology guidelines. Possible differences related to sex or sexual maturity were also investigated. The RIs for most of the analytes investigated were similar to those of other types of bats and other mammalian species. Haematology revealed mild polychromasia and slightly lower haematocrit, haemoglobin, leukocyte and lymphocyte counts compared to other bats. Glucose levels varied possibly due to stress, the anaesthetic protocol and fasting time. Creatine kinase was higher, while triglycerides were lower compared with domestic mammals and other bats. No sex- or age-related differences were found. Serum protein electrophoresis showed five fractions (albumin, α-, β₁-, β₂- and γ-globulins). The values recorded in this study could be helpful as a reference biological dataset to monitor the health status of wild and captive R. aegyptiacus and, possibly, of other Chiroptera.

蝙蝠作为许多具有人畜共患病潜力的新兴病毒性疾病的宿主，发挥着关键作用。然而，人们对蝙蝠，尤其是埃及果蝠 ( Rousettus aegyptiacus )的实验室参考区间 (RI) 知之甚少。本研究的目的是获得圈养果蝠的血液学、生化和电泳 RI。从 21 R. aegyptiacus收集血液（11 名女性和 10 名男性）。使用阻抗细胞计数器进行全血细胞计数，然后对血涂片进行形态学分析。临床生化用自动分光光度计进行，琼脂糖凝胶电泳用自动仪器进行。根据美国兽医临床病理学会指南，使用参考值顾问 V2.1 确定参考区间。还调查了与性别或性成熟相关的可能差异。所研究的大多数分析物的 RI 与其他类型的蝙蝠和其他哺乳动物物种的 RI 相似。与其他蝙蝠相比，血液学显示轻度多色症和略低的血细胞比容、血红蛋白、白细胞和淋巴细胞计数。血糖水平可能因压力而变化，麻醉方案和禁食时间。与家养哺乳动物和其他蝙蝠相比，肌酸激酶较高，而甘油三酯较低。未发现与性别或年龄相关的差异。血清蛋白电泳显示五个组分（白蛋白、α-、β₁ -、β ₂ - 和 γ-球蛋白）。本研究中记录的值可能有助于作为参考生物数据集，以监测野生和圈养的埃及罗汉果，可能还有其他手足目的健康状况。