Objective

Chondroprogenitors have recently gained prominence due to promising results seen in in vitro and animal studies as a potential contender in cell-based therapy for cartilage repair. Lack of consensus regarding nomenclature, isolation techniques, and expansion protocols create substantial limitations for translational research, especially given the absence of distinct markers of identification. The objective of this systematic review was to identify and collate information pertaining to hyaline cartilage–derived chondroprogenitors, with regard to their isolation, culture, and outcome measures.

Design

As per Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, a web-based search of Scopus and PubMed databases was performed from January 2000 to May 2020, which yielded 509 studies. A total of 65 studies were identified that met the standardized inclusion criteria which comprised of, but was not limited to, progenitors derived from fibronectin adhesion, migrated subpopulation from explant cultures, and single-cell sorting.

Result

Literature search revealed that progenitors demonstrated inherent chondrogenesis and minimal tendency for hypertrophy. Multiple sources also demonstrated significantly better outcomes that bone marrow–derived mesenchymal stem cells and comparable results to chondrocytes. With regard to progenitor subgroups, collated evidence points to better and consistent outcomes with the use of migratory progenitors when compared to fibronectin adhesion assay–derived progenitors, although a direct comparison between the two cell populations is warranted.

Conclusion

Since chondroprogenitors exhibit favorable properties for cartilage repair, efficient characterization of progenitors is imperative, to complete their phenotypic profile, so as to optimize their use in translational research for neocartilage formation.

中文翻译：

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思考透明软骨衍生的软骨祖细胞在组织再生中的潜力：系统评价。

客观的

由于在体外和动物研究中看到的有希望的结果，软骨祖细胞作为基于细胞的软骨修复疗法的潜在竞争者而备受关注。在命名法、分离技术和扩展方案方面缺乏共识给转化研究造成了很大的限制，尤其是在缺乏不同的识别标记的情况下。本系统评价的目的是识别和整理与透明软骨来源的软骨祖细胞有关的信息，包括它们的分离、培养和结果测量。

设计

根据系统评价和荟萃分析指南的首选报告项目，从 2000 年 1 月到 2020 年 5 月，对 Scopus 和 PubMed 数据库进行了基于网络的搜索，产生了 509 项研究。共确定了 65 项符合标准化纳入标准的研究，其中包括但不限于源自纤连蛋白粘附的祖细胞、来自外植体培养物的迁移亚群和单细胞分选。

结果

文献检索显示，祖细胞表现出固有的软骨形成和肥大的最小趋势。多个来源也显示出比骨髓间充质干细胞更好的结果以及与软骨细胞相当的结果。关于祖细胞亚组，与纤连蛋白粘附试验衍生的祖细胞相比，整理的证据表明使用迁移祖细胞具有更好和一致的结果，尽管有必要在两个细胞群之间进行直接比较。

结论

由于软骨祖细胞表现出对软骨修复的有利特性，因此必须对祖细胞进行有效表征，以完成其表型谱，从而优化它们在新软骨形成转化研究中的应用。