Over the past 8 years, the widespread adoption of CRISPR-based technologies has fueled the global genome editing revolution. This platform is based on Cas molecular machines such as Cas9, Cas12, Cas13, as well as other CRISPR effector proteins that are able to alter the genome, transcriptome, and epigenome of virtually any species. Technological improvements have rendered these tools more efficient and precise, and enabled functional diversification and specialization, as recently illustrated by the rise of base editing and the quickly growing demand for prime editing constructs. Here, we discuss the continued adoption of CRISPR tools and constructs distributed by the nonprofit organization Addgene, highlight the trends in the global demand for the CRISPR toolbox, and consider the widespread attitude changes around open sharing that are having a transformative effect on speeding up science.

中文翻译：

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与不断扩大的社区共享 CRISPR 工具箱。

在过去的 8 年中，基于 CRISPR 的技术的广泛采用推动了全球基因组编辑革命。该平台基于 Cas 分子机器，例如 Cas9、Cas12、Cas13，以及其他能够改变几乎任何物种的基因组、转录组和表观基因组的 CRISPR 效应蛋白。技术改进使这些工具更加高效和精确，并实现了功能的多样化和专业化，正如最近碱基编辑的兴起和对主要编辑结构的快速增长的需求所证明的那样。在这里，我们讨论了由非营利组织 Addgene 分发的 CRISPR 工具和结构的持续采用，强调了全球对 CRISPR 工具箱的需求趋势，