Somatic cell nuclear transfer (SCNT) is an important technique for biological science research. Cytoplasm injection cloning technology (CICT) was developed to improve the reprogramming efficiency as well as to overcome the limitations of SCNT. CICT uses an additional cytoplasm fused with an enucleated oocyte to restore the cytoplasmic volume of the cloned embryo, and this method could improve the reprogramming efficiency of the cloned embryo. In this study, we show that CICT can be adapted to mouse species to overcome the inefficiency of the SCNT method. In this study, results indicate that the two-cell embryo and blastocyst rates of cloned embryos with the use of the CICT method were significantly higher (p < 0.05) than that of the SCNT method (96.6% ± 1.1% vs. 86.7% ± 6.0%, 29.5% ± 2.6% vs. 22.1% ± 3.0%, respectively). Furthermore, the apoptotic cell number per blastocyst was significantly lower in the CICT group than that in the SCNT group (1.7 ± 0.2 vs. 2.9 ± 0.3, p < 0.05). Moreover, the acH3K9/K14 expression level in the CICT group was greater than that of the SCNT group (p < 0.05), and the relative acH3K56 level in the CICT group was significantly (p < 0.05) higher than that in the SCNT group. These results indicate that CICT helps improve the in vitro developmental competence and quality of cloned embryos.

中文翻译：

---

克隆胚胎的额外细胞质对小鼠体外发育能力和重编程效率的影响。

体细胞核移植（SCNT）是生物科学研究的重要技术。开发了细胞质注射克隆技术（CICT）以提高重编程效率并克服SCNT的局限性。CICT使用与去核卵母细胞融合的其他细胞质来恢复克隆胚胎的细胞质体积，这种方法可以提高克隆胚胎的重编程效率。在这项研究中，我们表明CICT可以适应小鼠物种，以克服SCNT方法的低效率。在这项研究中，结果表明，使用CICT方法克隆的胚胎的两细胞胚胎和胚泡率显着更高（p 小于SCNT方法的<0.05）（分别为96.6％±1.1％和86.7％±6.0％，29.5％±2.6％和22.1％±3.0％）。此外，CICT组的每个胚泡凋亡细胞数明显低于SCNT组（1.7±0.2 vs. 2.9±0.3，p  <0.05）。此外，CICT组的acH3K9 / K14表达水平高于SCNT组（p  <0.05），而CICT组的acH3K56相对水平显着（p  <0.05）高于SCNT组。这些结果表明，CICT有助于提高克隆胚胎的体外发育能力和质量。