A study was to investigate the regulation of bone marrow mesenchymal stem cells (BMSCs) on the stemness of hypopharyngeal cancer cells (FaDu cells). Green fluorescent protein-labeled FaDu cells were cocultured with BMSCs and then were isolated. In vitro experiments, including cell cycle and apoptosis analyses and clonogenic and sphere formation assays, were conducted using the cocultured FaDu cells to determine the stemness of FaDu cells. The tumor formation assay was performed through subcutaneous injection of FaDu cells into nude mice to determine the tumorigenic ability of FaDu cells after coculture. Immunohistochemical analysis of CD44 and ALDH1 was performed on the tumor tissue. After coculturing with human BMSCs, the ratio of FaDu cells at G2 phase was increased, while the ratios at S and G1 phases were decreased. In addition, coculture reduced apoptosis, but increased the clonogenic ability and sphere formation efficiency of FaDu cells. Finally, coculturing FaDu cells induced more robust and faster tumor formation as well as increased expression levels of CD44 and ALDH1 in tumor tissue. BMSCs promote the stemness of hypopharyngeal cancer cells.

中文翻译：

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骨髓间充质干细胞促进下咽癌细胞的干性。

一项研究旨在研究骨髓间充质干细胞（BMSCs）对下咽癌细胞（FaDu细胞）的干性的调节。将绿色荧光蛋白标记的FaDu细胞与BMSC共培养，然后分离。体外使用共培养的FaDu细胞进行了包括细胞周期和凋亡分析以及克隆形成和球形成测定在内的实验，以确定FaDu细胞的干性。通过将FaDu细胞皮下注射到裸鼠中进行肿瘤形成测定，以确定共培养后FaDu细胞的致瘤能力。在肿瘤组织上进行CD44和ALDH1的免疫组织化学分析。与人BMSCs共培养后，G2期FaDu细胞比例增加，而S和G1期FaDu细胞比例减少。另外，共培养减少了细胞凋亡，但是增加了FaDu细胞的克隆形成能力和球形成效率。最后，共培养FaDu细胞诱导更强壮和更快的肿瘤形成以及肿瘤组织中CD44和ALDH1的表达水平增加。