Mesenchymal stem cells (MSCs) have the potential to differentiate into neuron-like cells, which may provide a new strategy for the clinical treatment of neurodegenerative diseases such as Parkinson's disease (PD). However, the application of MSCs in the patients is still limited as the reason of efficiency and safety of transplantation. The aim of this study is to develop a new method and induce human umbilical cord MSCs (hUCMSCs) into neuron-like cells. Results from flow cytometry indicate that the isolated MSCs from hUCMSCs exhibited a typical phenotype of adult stem cells and express CD44, CD54, CD73, CD90, CD105, CD166, and HLA-ABC. Furthermore, the induced cells from hUCMSCs could spontaneously express different neural cell markers [neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP)], even transcription factors related to dopaminergic neuron's development (Nurr1, Wnt-1, and En-1). Moreover, after treatment of EHFBT (extracts of human fetal brain tissue), hUCMSCs can express neuronal markers such as Nestin, LIM homeobox transcription factor 1 beta (LMX1B), dopamine beta hydroxylase (DBH), and dopamine transporter (DAT). In summary, a method that can induce hUCMSCs into dopaminergic neuron containing cells is established in vitro by the treatment of EHFBT. This would provide us a new cell source for PD in clinical treatment in the future.

中文翻译：

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人胎脑提取物可诱导人脐带间充质干细胞向含有多巴胺能神经元的细胞分化。

间充质干细胞（MSCs）具有分化成神经元样细胞的潜力，这可能为神经退行性疾病如帕金森氏病（PD）的临床治疗提供新的策略。然而，由于移植效率和安全性的原因，MSC在患者中的应用仍然受到限制。这项研究的目的是开发一种新方法，并将人脐带间充质干细胞（hUCMSCs）诱导成神经元样细胞。流式细胞术的结果表明，从hUCMSC中分离出的MSC表现出成年干细胞的典型表型，并表达CD44，CD54，CD73，CD90，CD105，CD166和HLA-ABC。此外，来自hUCMSC的诱导细胞可以自发表达不同的神经细胞标志物[神经元特异性烯醇化酶（NSE）和神经胶质纤维酸性蛋白（GFAP）]，甚至与多巴胺能神经元发育有关的转录因子（Nurr1，Wnt-1和En-1）。此外，在治疗EHFBT（人类胎儿脑组织的提取物）后，hUCMSC可以表达神经元标记物，例如Nestin，LIM同源盒转录因子1 beta（LMX1B），多巴胺β羟化酶（DBH）和多巴胺转运蛋白（DAT）。总之，建立了一种可以诱导hUCMSCs进入含多巴胺能神经元细胞的方法在体外通过EHFBT的治疗。这将为我们将来在临床治疗中为PD提供新的细胞来源。