The effect of solvent type and extraction method was investigated to study the antioxidant and antibacterial activity of Polygonum minus. Two extraction methods were used: a solvent extraction using Soxhlet apparatus and supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using the ferric reducing/antioxidant power (FRAP) assay and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest polyphenol content was obtained from the medium polarity methanol extract of the leaf portion (645.60 ± 166.68 gallic acid equivalents/100 g (GAEs/100 g)). It also showed the highest antioxidant power for FRAP and DPPH radical inhibition and exhibited the largest inhibition zone in antibacterial activity on Bacillus subtilis (Gram+), Staphylococcus aureus (Gram+), and Escherichia coli (Gram−). The phase behavior and aldehyde profiles were further investigated using SFE with different cosolvents. The results indicated that a 50% ethanol-water cosolvent yielded the best aldehyde profiles in the presence of decanal, undecanal, and dodecanal.

中文翻译：

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Poly提取物的抗氧化和抗菌测定：不同提取方法

研究了溶剂种类和提取方法对虎杖的抗氧化和抗菌活性的影响。使用了两种萃取方法：使用索氏萃取仪的溶剂萃取和超临界流体萃取（SFE）。使用三价铁还原/抗氧化能力（FRAP）分析和2,2-二苯基-1-吡啶并肼基（DPPH）自由基清除能力评估抗氧化能力。从叶部分的中等极性甲醇提取物中可获得最高的多酚含量（645.60±166.68没食子酸当量/ 100 g（GAEs / 100 g））。它还对FRAP和DPPH自由基具有最高的抗氧化能力，对枯草芽孢杆菌（Gram +）的抗菌活性也表现出最大的抑制区，金黄色葡萄球菌（Gram +）和大肠杆菌（Gram-）。使用SFE和不同的助溶剂进一步研究了相行为和醛分布。结果表明，在存在癸醛，十一醛和十二醛的情况下，50％的乙醇-水助溶剂可产生最佳的醛谱。