Abstract

Background

Many food recalls are related to the presence of undeclared allergens and microorganisms in food products. To reduce these occurrences, portable diagnostic assay kits are available to quantitate mycotoxins, to detect allergens and gluten in foods and on environmental surfaces, and for sanitation monitoring.

Objective

This article reviews diagnostic kits that can detect sources of contamination in food and ingredients as well as on surfaces and clean-in-place rinses.

Method

Mycotoxins and gluten were detected using lateral flow diagnostic assays. Sanitation monitoring of surfaces was completed using a chemiluminescent assay to detect adenosine 5′-triphosphate disodium salt hydrate (ATP) and another assay to detect protein.

Results

Gluten was detected at 10 ppm in spiked commodities and on wet and dry surfaces at 2.5 µg/100cm². Deoxynivalenol was quantitated in dry distillers grains plus solubles and mean results were within two SDs of those determined by HPLC. The chemiluminescent assay had an LOD of 6 fmol of ATP and was able to detect a 1:10 000 dilution of orange juice from surfaces. The protein assay detected 5 µg of bovine serum albumin (BSA) directly applied to the sampler, 100 µg of BSA on surfaces, and detected 1:10 dilutions of Greek yogurt and raw beef from surfaces.

Conclusions

Portable diagnostic kits evaluated in this work provided accurate, rapid, and sensitive results for detection of mycotoxins, gluten, proteins, and ATP. These methods can be used in facilities with minimal training and provide results that are important to ensure food safety.

Highlights

Portable methods to detect gluten, mycotoxins, proteins, and ATP are presented.

中文翻译：

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用于检测霉菌毒素、过敏原和卫生监测的便携式分析仪概述

摘要

背景

许多食品召回与食品中存在未申报的过敏原和微生物有关。为减少此类事件的发生，可使用便携式诊断检测试剂盒来定量真菌毒素、检测食物和环境表面中的过敏原和麸质，以及用于卫生监测。

客观的

本文回顾了可以检测食品和成分中以及表面和原位清洁冲洗液中污染源的诊断试剂盒。

方法

霉菌毒素和面筋使用横向流动诊断测定法检测。使用化学发光测定法检测腺苷 5'-三磷酸二钠盐水合物 (ATP) 和另一种检测蛋白质的测定法完成表面的卫生监测。

结果

在加标商品中以及在干湿表面上检测到的面筋含量为 10 ppm，为 2.5 µg/100cm ²。在干酒糟和可溶物中对脱氧雪腐镰刀菌烯醇进行定量，平均结果与 HPLC 测定的结果在两个标准差范围内。化学发光分析的 LOD 为 6 fmol ATP，能够从表面检测到 1:10 000 稀释的橙汁。蛋白质测定检测到 5 µg 牛血清白蛋白 (BSA) 直接应用于采样器，100 µg BSA 在表面上，并从表面检测到希腊酸奶和生牛肉的 1:10 稀释度。

结论

在这项工作中评估的便携式诊断试剂盒为真菌毒素、麸质、蛋白质和 ATP 的检测提供了准确、快速和灵敏的结果。这些方法可以在接受最少培训的设施中使用，并提供对确保食品安全很重要的结果。

强调

介绍了检测面筋、霉菌毒素、蛋白质和 ATP 的便携式方法。