The present study evaluated the antifungal activity of the chelators deferiprone (DFP) and ethylenediaminetetraacetic acid (EDTA) and their effect on biofilm formation of the S. schenckii complex. Eighteen strains of Sporothrix spp. (seven S. brasiliensis, three S. globosa, three S. mexicana and five Sporothrix schenckii sensu stricto) were used. Minimum inhibitory concentration (MIC) values for EDTA and DFP against filamentous forms of Sporothrix spp. ranged from 32 to 128 μg/ml. For antifungal drugs, MIC values ranged from 0.25 to 4 μg/ml for amphotericin B, from 0.25 to 4 μg/ml for itraconazole, and from 0.03 to 0.25 μg/ml for terbinafine. The chelators caused inhibition of Sporothrix spp. in yeast form at concentrations ranging from 16 to 64 μg/ml (for EDTA) and 8 to 32 μg/ml (for DFP). For antifungal drugs, MIC values observed against the yeast varied from 0.03 to 0.5 μg/ml for AMB, 0.03 to 1 μg/ml for ITC, and 0.03 to 0.13 μg/ml for TRB. Both DFP and EDTA presented synergistic interaction with antifungals against Sporothrix spp. in both filamentous and yeast form. Biofilms formed in the presence of the chelators (512 μg/ml) showed a reduction of 47% in biomass and 45% in metabolic activity. Our data reveal that DFP and EDTA reduced the growth of planktonic cells of Sporothrix spp., had synergistic interaction with antifungal drugs against this pathogen, and reduced biofilm formation of Sporothrix spp.

中文翻译：

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去铁酮和 EDTA 对孢子丝菌的抗真菌活性：对浮游生长和生物膜形成的影响。


本研究评估了螯合剂去铁酮 (DFP) 和乙二胺四乙酸 (EDTA) 的抗真菌活性及其对申克链球菌复合物生物膜形成的影响。十八个孢子丝菌菌株。 （七个巴西链球菌、三个球形链球菌、三个墨西哥链球菌和五个申克孢子丝菌）被使用。 EDTA 和 DFP 对丝状孢子丝菌的最低抑制浓度 (MIC) 值。范围为 32 至 128 μg/ml。对于抗真菌药物，两性霉素 B 的 MIC 值范围为 0.25 至 4 μg/ml，伊曲康唑的 MIC 值范围为 0.25 至 4 μg/ml，特比萘芬的 MIC 值范围为 0.03 至 0.25 μg/ml。螯合剂引起孢子丝菌属的抑制。酵母形式，浓度范围为 16 至 64 μg/ml（对于 EDTA）和 8 至 32 μg/ml（对于 DFP）。对于抗真菌药物，针对酵母菌观察到的 MIC 值范围为：AMB 为 0.03 至 0.5 μg/ml，ITC 为 0.03 至 1 μg/ml，TRB 为 0.03 至 0.13 μg/ml。 DFP 和 EDTA 均与针对孢子丝菌的抗真菌药物具有协同相互作用。有丝状和酵母两种形式。在螯合剂 (512 μg/ml) 存在下形成的生物膜显示生物量减少了 47%，代谢活性减少了 45%。我们的数据表明，DFP 和 EDTA 减少了孢子丝菌属浮游细胞的生长，与针对该病原体的抗真菌药物具有协同相互作用，并减少了孢子丝菌属生物膜的形成。