Abstract Microsatellite markers are most valuable tools for characterization of plant genetic resources or population genetic analysis. Since they are codominant and allelic markers, utilizing them in polyploid species remained doubtful. In such cases, microsatellite markers are usually analyzed by treating them as dominant marker. In the current study, it has been showed that despite of losing the advantage of co-dominance, microsatellite markers are still powerful tool for genotyping of polyploid species because of availability of large number of reproducible alleles per locus. It has been studied by genotyping of nineteen sub populations of Dendrocalamus hamiltonii (hexaploid bamboo species) with seventeen polymorphic SSR primer pairs. Among these, ten primers gave typical banding pattern of microsatellite marker as expected in diploid species but rest seven gave unusual pattern i.e. more than two bands per locus per genotype. In such case genotyping data are generally analyzed by considering as dominant markers. Given these facts, data were analyzed in both ways as dominant and codominant. All the seventeen primer were first scored as non-allelic data and analyzed; later ten primer pairs giving standard banding pattern were analyzed as allelic data and the results were compared. The UPGMA clustering and genetic structure showed that results obtained with both the data sets were very similar, and therefore the SSR marker could be utilized to characterize polyploid species by considering them as dominant marker. The study is highly useful to widen the scope of SSR markers applications and beneficial to the researchers dealing with polyploid species.

中文翻译：

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微卫星标记在多倍体表征中的应用：以六倍体竹种为例

摘要 微卫星标记是表征植物遗传资源或群体遗传分析的最有价值的工具。由于它们是共显性和等位基因标记，因此在多倍体物种中利用它们仍然值得怀疑。在这种情况下，通常将微卫星标记作为显性标记进行分析。目前的研究表明，尽管失去了共显性优势，但微卫星标记仍然是多倍体物种基因分型的有力工具，因为每个基因座都有大量可重复的等位基因。通过对 Dendrocalamus hamiltonii（六倍体竹种）的 19 个亚群进行基因分型，使用 17 对多态 SSR 引物对其进行了研究。在这些当中，十个引物给出了二倍体物种中预期的微卫星标记的典型条带模式，但其余七个给出了不寻常的模式，即每个基因型每个基因座超过两个条带。在这种情况下，通常将基因分型数据视为显性标记进行分析。鉴于这些事实，数据以显性和共显性两种方式进行分析。所有17个引物首先作为非等位基因数据进行评分并进行分析；随后将给出标准条带模式的十个引物对作为等位基因数据进行分析并比较结果。UPGMA 聚类和遗传结构表明，两个数据集获得的结果非常相似，因此 SSR 标记可以通过将它们视为显性标记来表征多倍体物种。