Abstract

By using a mixed-ligand approach, a new Co(II)-bearing coordination polymer (CP) with the chemical formula of [{Co(BINDI)_0.5(bpa)_0.5(H₂O)}·4H₂O]_n (1, H₄BINDI = N,N′-bis(5-isophthalic acid)naphthalenediimide, bpa = 1,2-bis(4-pyridyl)ethane) has been synthesized by room temperature self-assembly and structurally characterized successfully. Furthermore, a process of sonochemistry with oleic acid treatment at 453 K was used to produce the nanostructure 1 (denoted as nano 1 hereafter). The inhibitory effect of nano 1 on cervical cancer cells was determined. Firstly, the CCK-8 assay was performed. The Reverse Transcription Polymerase Chain Reaction (RT-PCR) assay was performed and the relative expression levels of miR-16-1 were determined. Then, the western blotting detection was conducted and the Bcl-2 expression level after nano 1 treatment was evaluated.

抽象的

通过使用一种混合配体方法，一种新的化学式为[{Co（BINDI）_0.5（bpa）_0.5（H ₂ O）}·4H ₂ O] _n（如图1所示，通过室温自组装合成了H ₄ BINDI ＝N，N′-双（5-间苯二甲酸）萘二酰亚胺，bpa ＝ 1，2-双（4-吡啶基）乙烷，并成功地对其结构进行了表征。此外，使用在453 K下用油酸处理的声化学方法来制造纳米结构1（以下称为纳米1）。纳米1的抑制作用确定子宫颈癌细胞。首先，进行CCK-8测定。进行逆转录聚合酶链反应（RT-PCR）测定，并确定miR-16-1的相对表达水平。然后，进行蛋白质印迹检测并评估nano 1处理后的Bcl-2表达水平。